| A dynamic complex of signaling proteins uses polar localization to regulate cell-fate asymmetry in Caulobacter crescentus. | |
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MedLine Citation:
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PMID: 21397844 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cellular asymmetry is critical to metazoan development and the life cycle of many microbes. In Caulobacter, cell cycle progression and the formation of asymmetric daughter cells depend on the polarly-localized histidine kinase CckA. How CckA is regulated and why activity depends on localization are unknown. Here, we demonstrate that the unorthodox kinase DivL promotes CckA activity and that the phosphorylated regulator DivK inhibits CckA by binding to DivL. Early in the cell cycle, CckA is activated by the dephosphorylation of DivK throughout the cell. However, in later stages, when phosphorylated DivK levels are high, CckA activation relies on polar localization with a DivK phosphatase. Localization thus creates a protected zone for CckA within the cell, without the use of membrane-enclosed compartments. Our results reveal the mechanisms by which CckA is regulated in a cell-type-dependent manner. More generally, our findings reveal how cells exploit subcellular localization to orchestrate sophisticated regulatory processes. |
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Authors:
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Christos G Tsokos; Barrett S Perchuk; Michael T Laub |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental cell Volume: 20 ISSN: 1878-1551 ISO Abbreviation: Dev. Cell Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-14 Completed Date: 2011-05-12 Revised Date: 2012-04-26 |
Medline Journal Info:
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Nlm Unique ID: 101120028 Medline TA: Dev Cell Country: United States |
Other Details:
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Languages: eng Pagination: 329-41 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism* Caulobacter crescentus / cytology*, enzymology, physiology* Cell Cycle / physiology* Cell Polarity / physiology* Enzyme Activation Epistasis, Genetic Mutation Protein Kinases / genetics, metabolism* Recombinant Fusion Proteins / genetics, metabolism Signal Transduction / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM082899-03/GM/NIGMS NIH HHS; R01 GM082899-05/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DivK protein, Caulobacter crescentus; 0/Recombinant Fusion Proteins; EC 2.7.-/Protein Kinases; EC 2.7.3.-/protein-histidine kinase |
| Comments/Corrections | |
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