Document Detail


Duration and orientation of mechanical loads determine fibroblast cyto-mechanical activation: monitored by protease release.
MedLine Citation:
PMID:  10886814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regulation of mechanical force on and by connective tissues is increasingly regarded as a critical factor in understanding their function. The aim of this study was to identify quantifiable characteristics of external loading to which fibroblasts were sensitive. Specific patterns of uniaxial tensile loading were applied to fibroblast populated collagen lattices through a computer controlled driver: tensioning-Culture Force Monitor. Ramp loads of 120 dynes were applied over 10 minutes and 11 hours, and the effect of high and low strain pattern changes, with orientation relative to the applied load were investigated by using two lattice configurations, high and low aspect ratios, with loads applied across the long and short axis. Both, ramp loading and lattice orientation were tested in comparison to statically loaded gels. Changes in protease production were measured to indicate which loading patterns produced cell stimulation. Greatest cell responses were with the slowest rate of ramp loading and unloading (11 dynes/hour) with the 11 hour ramp causing a 5-fold increase in MMP-9 release. Similarly, the greatest stimulation was produced in high strain, aligned High Aspect Ratio lattices with a 2.6-fold increase in MMP-9 release. In conclusion, duration over which cells are loaded rather than the rate and alignment of that loading are both critical factors in cyto-mechanical activation.
Authors:
R T Prajapati; M Eastwood; R A Brown
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society     Volume:  8     ISSN:  1067-1927     ISO Abbreviation:  Wound Repair Regen     Publication Date:    2000 May-Jun
Date Detail:
Created Date:  2000-08-21     Completed Date:  2000-08-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9310939     Medline TA:  Wound Repair Regen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  238-46     Citation Subset:  IM; S    
Affiliation:
University College London, Division of Plastic and Reconstructive Surgery, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Endopeptidases / metabolism*
Fibroblasts / physiology*
Homeostasis / physiology
Humans
Matrix Metalloproteinases / metabolism
Middle Aged
Skin / cytology*
Stress, Mechanical
Urokinase-Type Plasminogen Activator / metabolism
Chemical
Reg. No./Substance:
EC 3.4.-/Endopeptidases; EC 3.4.21.73/Urokinase-Type Plasminogen Activator; EC 3.4.24.-/Matrix Metalloproteinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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