Document Detail


Duplication and suppression of chloroplast protein translocation genes in maize.
MedLine Citation:
PMID:  11139515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The HCF106 (high chlorophyll fluorescence) gene of maize encodes a chloroplast membrane protein required for translocation of a subset of proteins across the thylakoid membrane. Mutations in HCF106 caused by the insertion of Robertson's Mutator transposable elements have been mapped to chromosome 2S. Here we show that there is a closely related homolog of HCF106 encoded elsewhere in the maize genome (HCF106c) that can partially compensate for these mutations. This homolog maps on chromosome 10L and is part of the most recent set of segmental duplications in the maize genome. Triple mutants that are disrupted in both the HCF106 and Sec-dependent protein translocation pathways provide evidence that they act independently. The HCF106c gene accounts for a previously reported exception to the correlation between epigenetic suppression of hcf106 and methylation of Mutator transposons. We also demonstrate that insertions of Robertson's Mutator elements into either introns or promoters can lead to mutations whose phenotypes are suppressed in the absence of Mu activity, while alleles with insertions in both positions are not suppressed. The implications of these observations are discussed.
Authors:
A M Settles; A Baron; A Barkan; R A Martienssen
Related Documents :
14532095 - Development of a mariner-based transposon for use in sorangium cellulosum.
8454475 - The phylogeny of nine species of the drosophila obscura group inferred by the banding h...
2326275 - Constructing deletions with defined endpoints in drosophila.
9501115 - The transposition frequency of tag1 elements is increased in transgenic arabidopsis lines.
7438505 - A balanced translocation t(4;9) (q35;q12) with a breakpoint within the heterochromatic ...
12393285 - Different clones of t(1;12)/t(12;12) involving the etv6 gene in a case of acute myeloid...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  157     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-03-15     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  349-60     Citation Subset:  IM    
Affiliation:
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF237944;  AF237945
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Base Sequence
Biological Transport, Active / genetics
Chloroplasts / metabolism*
Cloning, Molecular
DNA Primers / genetics
DNA Transposable Elements / genetics
DNA, Plant / genetics
Gene Duplication
Genes, Plant*
Introns
Membrane Proteins / genetics*,  metabolism*
Models, Genetic
Molecular Sequence Data
Mutation
Phenotype
Plant Proteins / genetics*,  metabolism*
Sequence Homology, Amino Acid
Suppression, Genetic
Zea mays / genetics*,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 GM48179/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA Transposable Elements; 0/DNA, Plant; 0/Hcf106 protein, plant; 0/Membrane Proteins; 0/Plant Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Genetic and developmental analysis of X-inactivation in interspecific hybrid mice suggests a role fo...
Next Document:  Development and applications of a complete set of rice telotrisomics.