Document Detail


Duplex PCR of the Y-27H39 and HPRT loci with reference to Japanese population data on the HPRT locus.
MedLine Citation:
PMID:  9184015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using a duplex polymerase chain reaction (PCR) system, we successfully amplified GATA microsatellite loci Y-27H39 and HPRT on the Y and X chromosomes, respectively, and detected their alleles with a digoxigenin-labeled (GATA)6 probe. Among 450 unrelated Japanese including 225 females, we found 6 HPRT alleles ranging from 155 to 175 nucleotides with a simple repeat structure comprising 11 to 16 repeats. The duplex PCR is highly informative in Japanese.
Authors:
T Kishida; W Wang; M Fukuda; Y Tamaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nihon hōigaku zasshi = The Japanese journal of legal medicine     Volume:  51     ISSN:  0047-1887     ISO Abbreviation:  Nihon Hoigaku Zasshi     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-10-02     Completed Date:  1997-10-02     Revised Date:  2006-09-04    
Medline Journal Info:
Nlm Unique ID:  0413715     Medline TA:  Nihon Hoigaku Zasshi     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  67-9     Citation Subset:  IM    
Affiliation:
Department of Forensic Medicine, Oita Medical University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Asian Continental Ancestry Group
Female
Gene Frequency
Humans
Hypoxanthine Phosphoribosyltransferase / genetics*
Japan
Male
Microsatellite Repeats
Paternity
Polymerase Chain Reaction / methods
Polymorphism, Genetic
Sex Determination (Analysis)
X Chromosome
Y Chromosome*
Chemical
Reg. No./Substance:
EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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