| Duffy antigen expression on reticulocytes does not alter following blood loss in an autologous donation model. | |
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MedLine Citation:
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PMID: 19552696 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The Duffy blood group (Fy) antigen functions as the receptor whereby the malarial parasite Plasmodium vivax invades reticulocytes. In this study, we evaluated an autologous blood donation model to measure Fy expression during the anticipated response to blood loss. AIMS: This study aims to examine Fy expression following anticipated reticulocytosis in response to blood loss from autologous whole blood donation. METHOD: Subjects were healthy blood donors presenting for planned collection of two or three autologous units. Whole blood (450 ml +/- 10%) was collected and processed. Blood samples for Fy testing were obtained from the donations. These were assayed by flow cytometry by measuring binding of a phycoerythrin-labelled anti-Fy6 antibody and compared against reticulocyte numbers. Reticulocyte numbers were measured using thiazole orange. Results were compared from baseline (first donation) with samples at second and, if available, third, donations. Phenotyping for Fy a and b antigens was performed. RESULTS: Reticulocytes increased by a mean of 37% over baseline [0.93% (range 0.31-1.93) to 1.23% (0.32-3.51%)] following donation of two (n = 32) or three (n = 9) autologous whole blood units. Absolute reticulocyte count remained low. Mean and median Fy expression on mature red blood cells and reticulocytes did not change from baseline levels despite individual variation. No apparent relationship to serologically determined Fy a and/or b antigen status was present. CONCLUSION: Baseline expression of Fy antigen on mature red blood cells and reticulocytes is quite variable between individuals, but appears not to be greatly affected by mild to moderate reticulocytosis following blood loss in an autologous blood donation model. |
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Authors:
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I J Woolley; C M S Brown; P Hutchinson; V Turkou; K Visvanathan; E M Wood |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-22 |
Journal Detail:
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Title: Vox sanguinis Volume: 97 ISSN: 1423-0410 ISO Abbreviation: Vox Sang. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-28 Completed Date: 2010-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0413606 Medline TA: Vox Sang Country: England |
Other Details:
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Languages: eng Pagination: 268-72 Citation Subset: IM |
Affiliation:
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Centre for Inflammatory Diseases, Department of Infectious Diseases Monash Medical Centre, Monash University, Melbourne, Victoria, Australia. ian.woolley@med.monash.edu.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anemia / blood, complications Blood Donors* Blood Transfusion, Autologous Disease Susceptibility Duffy Blood-Group System / biosynthesis*, blood Female Humans Malaria, Vivax / blood Male Receptors, Cell Surface / biosynthesis*, blood Reticulocytes / metabolism* Reticulocytosis / immunology* |
| Chemical | |
Reg. No./Substance:
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0/DARC protein, human; 0/Duffy Blood-Group System; 0/Receptors, Cell Surface |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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