Document Detail


Duffy antigen expression on reticulocytes does not alter following blood loss in an autologous donation model.
MedLine Citation:
PMID:  19552696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The Duffy blood group (Fy) antigen functions as the receptor whereby the malarial parasite Plasmodium vivax invades reticulocytes. In this study, we evaluated an autologous blood donation model to measure Fy expression during the anticipated response to blood loss. AIMS: This study aims to examine Fy expression following anticipated reticulocytosis in response to blood loss from autologous whole blood donation. METHOD: Subjects were healthy blood donors presenting for planned collection of two or three autologous units. Whole blood (450 ml +/- 10%) was collected and processed. Blood samples for Fy testing were obtained from the donations. These were assayed by flow cytometry by measuring binding of a phycoerythrin-labelled anti-Fy6 antibody and compared against reticulocyte numbers. Reticulocyte numbers were measured using thiazole orange. Results were compared from baseline (first donation) with samples at second and, if available, third, donations. Phenotyping for Fy a and b antigens was performed. RESULTS: Reticulocytes increased by a mean of 37% over baseline [0.93% (range 0.31-1.93) to 1.23% (0.32-3.51%)] following donation of two (n = 32) or three (n = 9) autologous whole blood units. Absolute reticulocyte count remained low. Mean and median Fy expression on mature red blood cells and reticulocytes did not change from baseline levels despite individual variation. No apparent relationship to serologically determined Fy a and/or b antigen status was present. CONCLUSION: Baseline expression of Fy antigen on mature red blood cells and reticulocytes is quite variable between individuals, but appears not to be greatly affected by mild to moderate reticulocytosis following blood loss in an autologous blood donation model.
Authors:
I J Woolley; C M S Brown; P Hutchinson; V Turkou; K Visvanathan; E M Wood
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-22
Journal Detail:
Title:  Vox sanguinis     Volume:  97     ISSN:  1423-0410     ISO Abbreviation:  Vox Sang.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2010-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0413606     Medline TA:  Vox Sang     Country:  England    
Other Details:
Languages:  eng     Pagination:  268-72     Citation Subset:  IM    
Affiliation:
Centre for Inflammatory Diseases, Department of Infectious Diseases Monash Medical Centre, Monash University, Melbourne, Victoria, Australia. ian.woolley@med.monash.edu.au
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MeSH Terms
Descriptor/Qualifier:
Adult
Anemia / blood,  complications
Blood Donors*
Blood Transfusion, Autologous
Disease Susceptibility
Duffy Blood-Group System / biosynthesis*,  blood
Female
Humans
Malaria, Vivax / blood
Male
Receptors, Cell Surface / biosynthesis*,  blood
Reticulocytes / metabolism*
Reticulocytosis / immunology*
Chemical
Reg. No./Substance:
0/DARC protein, human; 0/Duffy Blood-Group System; 0/Receptors, Cell Surface

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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