Document Detail


Duddingtonia flagrans: centrifugal flotation technique with magnesium sulphate for the quantification and qualification of chlamydospores in sheep faeces.
MedLine Citation:
PMID:  18977351     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Duddingtonia flagrans is a nematode-trapping fungus responsible for attacking larval stages of helminths in pasture, which has potential as a biological control method. The aim of this study was to test the magnesium sulphate centrifugal flotation technique for the quantification of D. flagrans chlamydospores in sheep faeces and to verify their morphological viability. In this experiment one sheep received an oral dose of 4.5 x 10(6) chlamydospores/day during 20 days. Fecal samples were collected between days 15 and 20 and analyzed by the centrifugal flotation technique with magnesium sulphate. Densities of 1.23, 1.27 and 1.31gmL(-1) recovered 1.45 x 10(5), 3.87 x 10(5) and 1.65 x 10(5) chlamydospores from the faeces, respectively. Based upon the results it was concluded that this is an efficient technique for the chlamydospores quantification in ovine faeces. Moreover, it allowed more accurate visualization of chlamydospore morphology.
Authors:
Aleksandro Schafer da Silva; Régis Adriel Zanette; Mateus Anderson Otto; Cláudia Dal Molin Soares; Sydney Hartz Alves; Silvia Gonzalez Monteiro; Janio Morais Santurio
Publication Detail:
Type:  Journal Article     Date:  2008-10-17
Journal Detail:
Title:  Experimental parasitology     Volume:  121     ISSN:  1090-2449     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-02     Completed Date:  2009-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascomycota / growth & development*,  isolation & purification
Centrifugation
Feces / parasitology*
Filtration
Magnesium Sulfate / diagnostic use
Male
Sheep
Spores, Fungal / growth & development,  isolation & purification
Chemical
Reg. No./Substance:
7487-88-9/Magnesium Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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