Document Detail


Dual-wavelength fluorescent detection of particles on a novel microfluidic chip.
MedLine Citation:
PMID:  23291857     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This paper reports a novel lab-on-a-chip device that performs fluorescent particle counting by coupling the electrokinetically-induced pressure-driven flow and a miniaturized dual-wavelength fluorescent detection method. A novel L-shaped PDMS microchannel bonded on a thin glass slide is used to transport the particles. The dual-wavelength fluorescent detection system can count two different fluorescent particles simultaneously. Good agreement is achieved between the results obtained by the microfluidic chip device and the results from a commercial flow cytometer.
Authors:
Hai Jiang; Xuan Weng; Dongqing Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-7
Journal Detail:
Title:  Lab on a chip     Volume:  -     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1, Canada. dongqing@mme.uwaterloo.ca.
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