| Dual roles for macrophages in ovarian cycle-associated development and remodelling of the mammary gland epithelium. | |
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MedLine Citation:
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PMID: 21068060 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Each ovarian cycle, the mammary gland epithelium rotates through a sequence of hormonally regulated cell proliferation, differentiation and apoptosis. These studies investigate the role of macrophages in this cellular turnover. Macrophage populations and their spatial distribution were found to fluctuate across the cycle. The number of macrophages was highest at diestrus, and the greatest number of macrophages in direct contact with epithelial cells occurred at proestrus. The physiological necessity of macrophages in mammary gland morphogenesis during the estrous cycle was demonstrated in Cd11b-Dtr transgenic mice. Ovariectomised mice were treated with estradiol and progesterone to stimulate alveolar development, and with the progesterone receptor antagonist mifepristone to induce regression of the newly formed alveolar buds. Macrophage depletion during alveolar development resulted in a reduction in both ductal epithelial cell proliferation and the number of alveolar buds. Macrophage depletion during alveolar regression resulted in an increased number of branch points and an accumulation of TUNEL-positive cells. These studies show that macrophages have two roles in the cellular turnover of epithelial cells in the cycling mammary gland; following ovulation, they promote the development of alveolar buds in preparation for possible pregnancy, and they remodel the tissue back to its basic architecture in preparation for a new estrous cycle. |
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Authors:
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Angela C L Chua; Leigh J Hodson; Lachlan M Moldenhauer; Sarah A Robertson; Wendy V Ingman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-10 |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 137 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2010-12-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 4229-38 Citation Subset: IM |
Affiliation:
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The Robinson Institute, Research Centre for Reproductive Health, and School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide 5005, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Epithelium / drug effects, metabolism* Estradiol / blood, pharmacology Estrogens / pharmacology Estrous Cycle / drug effects, physiology* Female Flow Cytometry Hormone Antagonists / pharmacology Immunohistochemistry In Situ Nick-End Labeling Macrophages / metabolism* Mammary Glands, Animal / cytology*, drug effects, metabolism* Mice Mice, Inbred C57BL Mifepristone / pharmacology Progesterone / blood, pharmacology Progestins / pharmacology Receptors, Progesterone / antagonists & inhibitors |
| Chemical | |
Reg. No./Substance:
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0/Estrogens; 0/Hormone Antagonists; 0/Progestins; 0/Receptors, Progesterone; 50-28-2/Estradiol; 57-83-0/Progesterone; 84371-65-3/Mifepristone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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