Document Detail

Dual role for Drosophila lethal of scute in CNS midline precursor formation and dopaminergic neuron and motoneuron cell fate.
MedLine Citation:
PMID:  21558367     Owner:  NLM     Status:  MEDLINE    
Dopaminergic neurons play important behavioral roles in locomotion, reward and aggression. The Drosophila H-cell is a dopaminergic neuron that resides at the midline of the ventral nerve cord. Both the H-cell and the glutamatergic H-cell sib are the asymmetric progeny of the MP3 midline precursor cell. H-cell sib cell fate is dependent on Notch signaling, whereas H-cell fate is Notch independent. Genetic analysis of genes that could potentially regulate H-cell fate revealed that the lethal of scute [l(1)sc], tailup and SoxNeuro transcription factor genes act together to control H-cell gene expression. The l(1)sc bHLH gene is required for all H-cell-specific gene transcription, whereas tailup acts in parallel to l(1)sc and controls genes involved in dopamine metabolism. SoxNeuro functions downstream of l(1)sc and controls expression of a peptide neurotransmitter receptor gene. The role of l(1)sc may be more widespread, as a l(1)sc mutant shows reductions in gene expression in non-midline dopaminergic neurons. In addition, l(1)sc mutant embryos possess defects in the formation of MP4-6 midline precursor and the median neuroblast stem cell, revealing a proneural role for l(1)sc in midline cells. The Notch-dependent progeny of MP4-6 are the mVUM motoneurons, and these cells also require l(1)sc for mVUM-specific gene expression. Thus, l(1)sc plays an important regulatory role in both neurogenesis and specifying dopaminergic neuron and motoneuron identities.
Stephanie B Stagg; Amaris R Guardiola; Stephen T Crews
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  138     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-11     Completed Date:  2011-07-21     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2171-83     Citation Subset:  IM    
Curriculum in Neurobiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA.
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MeSH Terms
Basic Helix-Loop-Helix Transcription Factors / genetics,  metabolism*
Central Nervous System / embryology
DNA-Binding Proteins / genetics,  metabolism
Dopamine / metabolism
Drosophila / cytology,  embryology*,  genetics
Drosophila Proteins / genetics,  metabolism*
Gene Expression Regulation, Developmental*
In Situ Hybridization
Motor Neurons / cytology,  physiology*
Neurons / cytology,  physiology*
Receptors, Neurotransmitter / biosynthesis,  genetics
Receptors, Notch / metabolism
SOX Transcription Factors / genetics,  metabolism
Signal Transduction
Transcription Factors / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/DNA-Binding Proteins; 0/Drosophila Proteins; 0/Receptors, Neurotransmitter; 0/Receptors, Notch; 0/SOX Transcription Factors; 0/SoxNeuro protein, Drosophila; 0/Transcription Factors; 0/achaete protein, Drosophila; 0/islet protein, Drosophila; 0/lethal of scute protein, Drosophila; 0/scute protein, Drosophila

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