Document Detail

Dual plane in-line digital holographic microscopy.
MedLine Citation:
PMID:  20967088     Owner:  NLM     Status:  MEDLINE    
We report a dual plane in-line digital holographic microscopy technique that exploits the method of subtraction of average intensity of the entire hologram to suppress the zero-order diffracted wave. Two interferograms are recorded at different planes to eliminate the conjugate image. The experimental results demonstrate successful reconstruction of phase objects as well as of amplitude objects. The two interferograms can be recorded simultaneously, using two CCD or CMOS sensors, in order to increase the acquisition rate. This enhanced acquisition rate, together with the improved reconstruction capability of the proposed method, may find applications in biomedical research for visualization of rapid dynamic processes at the cellular level.
Bhargab Das; Chandra S Yelleswarapu
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Publication Detail:
Type:  Letter; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Optics letters     Volume:  35     ISSN:  1539-4794     ISO Abbreviation:  Opt Lett     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-22     Completed Date:  2011-02-17     Revised Date:  2012-08-27    
Medline Journal Info:
Nlm Unique ID:  7708433     Medline TA:  Opt Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3426-8     Citation Subset:  IM    
Department of Physics, University of Massachusetts, Boston, Massachusetts 02125, USA.
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MeSH Terms
Equipment Design
Holography / methods*
Image Enhancement / methods
Image Interpretation, Computer-Assisted / methods
Image Processing, Computer-Assisted / methods*
Interferometry / methods
Microscopy / methods*
Signal Processing, Computer-Assisted
Grant Support
1R21RR024429-01A1/RR/NCRR NIH HHS; R21 RR024429/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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