Document Detail

Dual-modality optical and positron emission tomography imaging of vascular endothelial growth factor receptor on tumor vasculature using quantum dots.
MedLine Citation:
PMID:  18566815     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To date, the in vivo imaging of quantum dots (QDs) has been mostly qualitative or semiquantitative. The development of a dual-function positron emission tomography (PET)/near-infrared fluorescence (NIRF) probe might allow the accurate assessment of the tumor-targeting efficacy of QDs. MATERIALS AND METHODS: An amine-functionalized QD was conjugated with VEGF protein and DOTA chelator for VEGFR-targeted PET/NIRF imaging after (64)Cu-labeling. The targeting efficacy of this dual functional probe was evaluated in vitro and in vivo through cell-binding assay, cell staining, in vivo optical/PET imaging, ex vivo optical/PET imaging, and histology. RESULTS: The DOTA-QD-VEGF exhibited VEGFR-specific binding in both cell-binding assay and cell staining experiment. Both NIR fluorescence imaging and microPET showed VEGFR-specific delivery of conjugated DOTA-QD-VEGF nanoparticle and prominent reticuloendothelial system uptake. The U87MG tumor uptake of (64)Cu-labeled DOTA-QD was less than one percentage injected dose per gram (%ID/g), significantly lower than that of (64)Cu-labeled DOTA-QD-VEGF (1.52 +/- 0.6%ID/g, 2.81 +/- 0.3%ID/g, 3.84 +/- 0.4%ID/g, and 4.16 +/- 0.5%ID/g at 1, 4, 16, and 24 h post injection, respectively; n = 3). Good correlation was also observed between the results measured by ex vivo PET and NIRF organ imaging. Histologic examination revealed that DOTA-QD-VEGF primarily targets the tumor vasculature through a VEGF-VEGFR interaction. CONCLUSION: We have successfully developed a QD-based nanoprobe for dual PET and NIRF imaging of tumor VEGFR expression. The success of this bifunctional imaging approach may render higher degree of accuracy for the quantitative targeted NIRF imaging in deep tissue.
Kai Chen; Zi-Bo Li; Hui Wang; Weibo Cai; Xiaoyuan Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-06-20
Journal Detail:
Title:  European journal of nuclear medicine and molecular imaging     Volume:  35     ISSN:  1619-7089     ISO Abbreviation:  Eur. J. Nucl. Med. Mol. Imaging     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-03-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101140988     Medline TA:  Eur J Nucl Med Mol Imaging     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2235-44     Citation Subset:  IM    
Department of Radiology and Bio-X Program, School of Medicine, Stanford University, Stanford, CA 94305-5484, USA.
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MeSH Terms
Amines / chemistry
Chelating Agents / chemistry
Copper Radioisotopes / chemistry
Fluorescent Antibody Technique
Fluorescent Dyes / chemical synthesis,  chemistry
Frozen Sections
Heterocyclic Compounds, 1-Ring / chemistry
Infrared Rays
Neoplasms / blood supply*,  pathology,  radionuclide imaging*
Positron-Emission Tomography
Quantum Dots*
Receptors, Vascular Endothelial Growth Factor / metabolism*
Staining and Labeling
Tomography, Optical
Vascular Endothelial Growth Factor A / chemistry,  metabolism
Grant Support
P50 CA114747/CA/NCI NIH HHS; R01 CA119053/CA/NCI NIH HHS; R21 CA102123/CA/NCI NIH HHS; R21 CA121842/CA/NCI NIH HHS; R24 CA93862/CA/NCI NIH HHS; U54 CA119367/CA/NCI NIH HHS
Reg. No./Substance:
0/Amines; 0/Chelating Agents; 0/Copper Radioisotopes; 0/Fluorescent Dyes; 0/Heterocyclic Compounds, 1-Ring; 0/Vascular Endothelial Growth Factor A; 60239-18-1/1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; EC, Vascular Endothelial Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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