| Dual kinase inhibition promotes pluripotency in finite bovine embryonic cell lines. | |
| | |
MedLine Citation:
|
PMID: 23282176 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Embryonic pluripotent stem cells (ePSCs) can generate all somatic cell types, as well as functional gametes. In mouse and rat, derivation of ePSCs from the early epiblast is promoted by the double inhibition ("2i") of mitogen-activated protein kinase kinase (MAP2K), antagonizing FGF signaling, and glycogen synthase kinase 3 (GSK3), stimulating the WNT pathway. However, it has remained unclear whether this culture regime is applicable to non-rodent livestock species. Here, we report the generation of bovine ePSCs under minimal conditions. Inner cell masses (ICMs) were immunosurgically isolated from in vitro fertilized bovine blastocysts and cultured feeder-free in 2i medium. Dual kinase inhibition primed bovine ICMs for stem cell derivation and sustained expression of epiblast-specific pluripotency markers SOX2 and NANOG, while repressing hypoblast marker GATA4. Following mechanical passage, 2i supported limited proliferation for several weeks. Continuously cultured ePSC lines expressed discriminatory markers of naïve pluripotency and primordial germ cells, but not of primed epiblast stem cells. In female ePSCs, most OCT4-positive cells lacked epigenetically silenced X-chromosomes, displaying a diagnostic feature of naïve pluripotency. Bovine ePSCs maintained a normal karyotype and differentiated into derivatives of all three germ layers in suspension culture. This culture system provides a screening platform for factors that maintain long-term proliferation of pluripotent embryonic cattle cells without genetic intervention. |
| | |
Authors:
|
Vinod Verma; Ben Huang; Prasanna Kumar Kallingappa; Bjorn Oback |
Related Documents
:
|
22924666 - Contribution of endothelial cells to human bone derived cells expansion in co-culture. 23668786 - The effects of human umbilical cord perivascular cells on rat hepatocyte structure and ... 11319276 - Human neuroblastoma cell viability and growth are affected by altered culture conditions. 15058196 - Cell growth and division processes are differentially sensitive to cadmium in scenedesm... 12700186 - Apoptosis and in vitro development of preimplantation porcine embryos derived in vitro ... 1789996 - Differences of metabolism of prostaglandin e2 and f2 alpha by decidual stromal cells an... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-2 |
Journal Detail:
|
Title: Stem cells and development Volume: - ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
National Heart Centre Singapore, Research and Development Unit, Singapore, Singapore; vinodverma29@rediffmail.com. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ligand access to the active site in Thermus thermophilus ba(3) and bovine heart aa(3) cytochrome oxi...
Next Document: Species-level classification of the vaginal microbiome.