Document Detail

Dual effects of tumor necrosis factor alpha on myocardial injury following prolonged hypoperfusion of the heart.
MedLine Citation:
PMID:  24949667     Owner:  NLM     Status:  Publisher    
Objective: To examine the dose response of TNFα in an ex vivo rat model of myocardial ischemia reperfusion. Methods and Results: Seventy-two rat hearts were mounted on Langendorff apparatus and perfused with oxygenated Krebs-Henseleit solutions. Ischemia was induced by reducing the perfusate flow rate. During reperfusion, incremental doses of recombinant TNFα were infused as a part of perfusate. TNFα was blocked with monoclonal TNFα antibody. Myocardial function was measured by dP/dT and relaxation time (IVRT). Cellular injury was assessed by released myoglobin and tissue concentration of malondialdehyde activity of the heart homogenates. Baseline +dP/dT was 1645 ± 125 mmHg/sec, -dP/dT was 945 ± 73 mmHg/sec and IVRT was 65 ± 5 msec. At the conclusion of reperfusion period, lower doses of TNFα increased +dP/dT and lowered IVRT. In contrast, the higher doses of TNFα decreased +dP/dT and prolonged IVRT. Pretreating the hearts with monoclonal TNFα antibody completely abolished the effects of TNFα on myocardial contractility and relaxation comparable to ischemia controls. Conclusion: Low dose TNFα improved myocardial function and decreased resultant cellular injury while high dose TNFα decreased myocardial function and increased myocardial injury following ischemia and reperfusion.
Mehrdad Asgeri; Leili Pourafkari; Amita Kundra; Hassan Javadzadegan; Sohrab Negargar; Nader D Nader
Related Documents :
9504727 - Analysis of left ventricular function after emergency coronary artery bypass grafting f...
19081647 - Incident coronary revascularization and subsequent mortality in chronic heart failure: ...
24949667 - Dual effects of tumor necrosis factor alpha on myocardial injury following prolonged hy...
1541447 - Percutaneous coronary intervention in the 1990s. results in patients with single or mul...
18264747 - Diastolic dysfunction and atrial fibrillation.
8874777 - Regional distribution of hsp70 proteins after myocardial infarction.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-20
Journal Detail:
Title:  Immunological investigations     Volume:  -     ISSN:  1532-4311     ISO Abbreviation:  Immunol. Invest.     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8504629     Medline TA:  Immunol Invest     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-13     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Atmospheric Gas Phase Chemistry of CH2=NH and HNC. A First Principles Approach.
Next Document:  Reflections on the theories of aging, of oxidative stress and of science in general. Is it time to a...