Document Detail

Dual effects of folinic acid in 5-fluorouracil induced killing of human tumor cell lines in vitro.
MedLine Citation:
PMID:  8917376     Owner:  NLM     Status:  MEDLINE    
Folinic acid (leucovorin) is frequently used to augment and modulate the clinical activity of 5-fluorouracil (5-FU) in patients with advanced gastrointestinal (Gl) cancer. However, there are conflicting opinions concerning the optimal doses for these patients, and whether folinic acid modulates the clinical activity of 5-FU in patients with non-Gl cancer. To elucidate these questions, model experiments have been performed on human tumor cell lines in vitro to determine the modulatory activity of various concentrations of folinic acid on 5-FU mediated cytotoxicity using a clonogenic assay. Three cell lines of colon cancer and 3 of glioblastoma origin were exposed to 5-FU alone or with folinic acid for 24 hours. It was observed that relatively low concentrations of folinic acid enhanced the cytotoxicity of 5-FU against the colon cancer lines whereas higher concentrations were less effective. Folinic acid did not enhance the 5-FU mediated killing of the glioma cell lines at any concentration (0.01-100 micrograms/ml). On the contrary, folinic acid seemed to counteract the cytotoxic effect of 5-FU in a reasonably dose-dependent fashion. These results may suggest that the value of folinic acid in the treatment of non-Gl cancer with 5-FU should be evaluated within the framework of controlled clinical trials, and that high doses of folinic acid may not necessarily be more effective than low.
H Blomgren; D Edler; M Hallström; P Ragnhammar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  16     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1996 Sep-Oct
Date Detail:
Created Date:  1996-12-18     Completed Date:  1996-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  2713-7     Citation Subset:  IM    
Karolinska Hospital, Stockholm, Sweden.
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MeSH Terms
Antimetabolites, Antineoplastic / pharmacology*
Colonic Neoplasms
Drug Synergism
Fluorouracil / pharmacology*
Leucovorin / administration & dosage,  pharmacology*
Tumor Cells, Cultured / drug effects
Tumor Stem Cell Assay
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 51-21-8/Fluorouracil; 58-05-9/Leucovorin

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