Document Detail

Dual effect of osmotic cell swelling on prolactin secretion by acutely dispersed adenohypophyseal cells.
MedLine Citation:
PMID:  1899279     Owner:  NLM     Status:  MEDLINE    
Cell swelling induced by acute exposure to the permeant molecule urea or by medium hyposmolarity evoked a prompt PRL secretory burst from dispersed rat anterior pituitary cells. However, during continuous exposure greater than or equal to 10 min to these conditions inhibition of basal and TRH-induced PRL secretion occurred and there was an "off" burst of PRL secretion following return to basal conditions. Compared with continuous TRH stimulation which causes biphasic PRL secretion with a rapid high amplitude first phase secretory burst followed by a sustained low level second phase of secretion, cell swelling induced only "first phase" secretion. Removing Ca2+ from the medium or adding 50 microM verapamil markedly depressed the "off" secretory burst following return to basal conditions but had no effect on the initial high amplitude burst. Our data suggest that the effect of cell swelling on PRL secretion is complex and that there are at least two mechanisms for PRL secretion in normal anterior pituitary cells; these are differently affected by cell swelling and Ca2+ influx.
X B Wang; N Sato; M A Greer; S McAdams; S E Greer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Life sciences     Volume:  48     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1991  
Date Detail:
Created Date:  1991-03-07     Completed Date:  1991-03-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  617-22     Citation Subset:  IM    
Department of Medicine, Oregon Health Sciences University, Portland 97201.
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MeSH Terms
Calcium / pharmacology
Egtazic Acid / pharmacology
Hypotonic Solutions
Pituitary Gland, Anterior / cytology,  secretion*
Prolactin / secretion*
Rats, Inbred Strains
Thyrotropin-Releasing Hormone / pharmacology
Urea / pharmacology
Verapamil / pharmacology
Grant Support
Reg. No./Substance:
0/Hypotonic Solutions; 24305-27-9/Thyrotropin-Releasing Hormone; 52-53-9/Verapamil; 57-13-6/Urea; 67-42-5/Egtazic Acid; 7440-70-2/Calcium; 9002-62-4/Prolactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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