Document Detail

Dual effect of nerve growth factor on cell death of PC12 cells Induced by serum deprivation.
MedLine Citation:
PMID:  12018837     Owner:  NLM     Status:  MEDLINE    
The effect of nerve growth factor (NGF) on the cell death of PC12 cells that is induced by serum deprivation was examined in the floating and attached cells to the extracellular matrix. NGF suppressed cell death occurred in the floating cells. The onset of cell death in the attached cells was much slower than in the floating cells. Moreover, the cell death in the attached cells was either accelerated in a high-density culture (over approximately 50% confluent), or inhibited in a low-density culture by NGF. While nucleosomal DNA fragmentation and poly (ADP-ribose) polymerase degradation was observed in both the floating and attached cells, the incidence of nuclear fragmentation and chromatin condensation was much lower in the attached cells than in the floating cells. The delayed onset of cell death in the attached cells was due to the signals that are generated from the extracellular matrix that is formed by PC12 cells, together with cell-to-cell interaction. The acceleration of cell death in the NGF-treated cells was anoikis, caused by the loss of the anchorage of the cell via the action of increased activities of matrix metalloproteinases (MMP2, MMP9). These results suggest that NGF has a different role in the cell death of PC12 cells that is induced by serum deprivation, depending on the cell-matrix, as well as the cell-cell interaction.
Mijung Kwon; Sooryun Seo; Hoyoung Chun; Jun-Mo Chung; In Kwon Chung; Kyu Chung Hur
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  13     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-05-20     Completed Date:  2003-01-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  167-74     Citation Subset:  IM    
Department of Biology and Center for Cellular Signaling Research, Ewha Womans University, Seoul, Korea.
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MeSH Terms
Cell Adhesion / physiology
Cell Count
Cell Death / drug effects*
Cell Size
Culture Media, Serum-Free*
DNA Fragmentation
Nerve Growth Factor / pharmacology*
Nucleosomes / genetics
PC12 Cells
Poly(ADP-ribose) Polymerases
Proteins / metabolism
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Nucleosomes; 0/Proteins; 9061-61-4/Nerve Growth Factor; EC protein, rat; EC Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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