| Dual effect of 2-methoxyestradiol on cell cycle events in human osteosarcoma 143B cells. | |
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MedLine Citation:
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PMID: 12136957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have demonstrated for the first time that the steroid metabolite, 2-methoxyestradiol (2-ME) is a powerful growth inhibitor of human osteosarcoma 143 B cell line by pleiotropic mechanisms involving cell cycle arrest at two different points and apoptosis. The ability of 2-ME to inhibit cell cycle at the respective points has been found concentration dependent. 1 microM 2-ME inhibited cell cycle at G1 phase while 10 microM 2-ME caused G2/M cell cycle arrest. As a natural estrogen metabolite 2-ME is expected to perturb the stability of microtubules (MT) in vivo analogously to Taxol--the MT binding anticancer agent. Contrary to 2-ME, Taxol induced accumulation of osteosarcoma cells in G2/M phase of cell cycle only. The presented data strongly suggest two different mechanisms of cytotoxic action of 2-ME at the level of a single cell. |
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Authors:
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Justyna Gołebiewska; Piotr Rozwadowski; Jan Henryk Spodnik; Narcyz Knap; Takashi Wakabayashi; Michał Woźniak |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta biochimica Polonica Volume: 49 ISSN: 0001-527X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-07-24 Completed Date: 2003-01-22 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: Poland |
Other Details:
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Languages: eng Pagination: 59-65 Citation Subset: IM |
Affiliation:
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Department of Medical Chemistry, Medical University of Gdańsk, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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physiology* Estradiol / analogs & derivatives*, pharmacology* Humans Microscopy, Confocal Microtubules / metabolism Osteosarcoma / drug therapy* Tubulin / metabolism Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Tubulin; 362-07-2/2-methoxyestradiol; 50-28-2/Estradiol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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