Document Detail


Dual asymmetric-flow microdialysis for in vivo monitoring of brain neurochemicals.
MedLine Citation:
PMID:  21872041     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Microdialysis is an extensively used technique for both in vivo and in vitro experiments, applicable to animal and human studies. In neurosciences, the in vivo microdialysis is usually performed to follow changes in the extracellular levels of substances and to monitor neurotransmitters release in the brain of freely moving animals. Catecholamines, such as dopamine and their related compounds, are involved in the neurochemistry and in the physiology of mental diseases and neurological disorders. It is generally supposed that the brain's energy requirement is supplied by glucose oxidation. More recently, lactate was proposed to be the metabolic substrate used by neurons during synaptic activity. In our study, an innovative microdialysis approach for simultaneous monitoring of catecholamines, indolamines, glutamate and energy substrates in the striatum of freely moving rats, using an asymmetric perfusion flow rate on microdialysis probe, is described. As a result of this asymmetric perfusion, two samples are available from the same brain region, having the same analytes composition but different concentrations. The asymmetric flow perfusion could be a useful tool in neurosciences studies related to brain's energy requirement, such as toxin-induced models of Parkinson's disease.
Authors:
Gianfranco Bazzu; Alice Biosa; Donatella Farina; Ylenia Spissu; Sonia Dedola; Giammario Calia; Giulia Puggioni; Gaia Rocchitta; Rossana Migheli; Maria Speranza Desole; Pier Andrea Serra
Publication Detail:
Type:  Journal Article     Date:  2011-07-18
Journal Detail:
Title:  Talanta     Volume:  85     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1933-40     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Department of Neuroscience, Medical School, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, Italy.
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