Document Detail


Dual-surface modified virus capsids for targeted delivery of photodynamic agents to cancer cells.
MedLine Citation:
PMID:  20863095     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteriophage MS2 was used to construct a targeted, multivalent photodynamic therapy vehicle for the treatment of Jurkat leukemia T cells. The self-assembling spherical virus capsid was modified on the interior surface with up to 180 porphyrins capable of generating cytotoxic singlet oxygen upon illumination. The exterior of the capsid was modified with ∼20 copies of a Jurkat-specific aptamer using an oxidative coupling reaction targeting an unnatural amino acid. The capsids were able to target and selectively kill more than 76% of the Jurkat cells after only 20 min of illumination. Capsids modified with a control DNA strand did not target Jurkat cells, and capsids modified with the aptamer were found to be specific for Jurkat cells over U266 cells (a control B cell line). The doubly modified capsids were also able to kill Jurkat cells selectively even when mixed with erythrocytes, suggesting the possibility of using our system to target blood-borne cancers or other pathogens in the blood supply.
Authors:
Nicholas Stephanopoulos; Gary J Tong; Sonny C Hsiao; Matthew B Francis
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  ACS nano     Volume:  4     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-26     Completed Date:  2011-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6014-20     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of California, Berkeley, and Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, California 94720-1460, USA.
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MeSH Terms
Descriptor/Qualifier:
Aptamers, Nucleotide
B-Lymphocytes / metabolism
Capsid / chemistry*
Capsid Proteins / chemistry*
Cell Line
Drug Delivery Systems
Erythrocytes / cytology
Humans
Jurkat Cells
Levivirus / metabolism
Neoplasms / pathology,  radiotherapy*
Photochemotherapy / methods*
Porphyrins
Singlet Oxygen / chemistry
Spectrophotometry / methods
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 0/Capsid Proteins; 0/Porphyrins; 17778-80-2/Singlet Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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