Document Detail


DuPont Qualicon BAX System assay for genus Listeria 24E.
MedLine Citation:
PMID:  21797015     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The new BAX System PCR Assay for Genus Listeria 24E was evaluated for detecting Listeria spp. in frankfurters, spinach, cooked shrimp, queso fresco cheese, and on stainless steel surfaces with a single-stage enrichment in BAX System 24 Listeria Enrichment Broth (24 LEB). Method comparison studies performed on samples with low-level inoculates showed that the BAX System demonstrates a sensitivity equivalent or superior to the U.S. Food and Drug Administration's Bacteriological Analytical Manual and the U.S. Department of Agriculture-Food Safety and Inspection Service culture methods, but with a significantly shorter time to result. Tests to evaluate inclusivity and exclusivity returned no false-negative and no false-positive results on a diverse panel of isolates, and tests for lot-to-lot variability and tablet stability demonstrated consistent performance. Ruggedness studies determined that none of the factors examined, within the range of deviations from specified parameters examined, affect the performance of the assay.
Authors:
F Morgan Wallace; Dawn Fallon; Daniel DeMarco; Stephen Varkey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  94     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2011 May-Jun
Date Detail:
Created Date:  2011-07-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  863-71     Citation Subset:  IM    
Affiliation:
DuPont Qualicon, Rt. 141 and Henry Clay Rd, DuPont Experimental Station, Wilmington, DE 19880-0400, USA. morgan.wallace@usa.dupont.com
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