Document Detail


DsbA plays a critical and multifaceted role in the production of secreted virulence factors by the phytopathogen Erwinia carotovora subsp. atroseptica.
MedLine Citation:
PMID:  18562317     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erwinia carotovora subsp. atroseptica is an enterobacterial phytopathogen causing economically significant soft rot disease. Pathogenesis is mediated by multiple secreted virulence factors, many of which are secreted by the type II (Out) secretion system. DsbA catalyzes the introduction of disulfide bonds into periplasmic and secreted proteins. In this study, the extracellular proteome (secretome) of wild type E. carotovora subsp. atroseptica SCRI1043, and dsbA and out mutants, was analyzed by spectral counting mass spectrometry. This revealed that dsbA inactivation had a huge impact on the secretome and identified diverse DsbA- and Out-dependent secreted proteins, representing known, predicted, and novel candidate virulence factors. Further characterization of the dsbA mutant showed that secreted enzyme activities, motility, production of the quorum-sensing signal, and virulence were absent or substantially reduced. The impact of DsbA on secreted virulence factor production was mediated at multiple levels, including impacting on the Out secretion system and the virulence gene regulatory network. Transcriptome analyses revealed that the abundance of a broad, but defined, set of transcripts, including many virulence factors, was altered in the dsbA mutant, identifying a new virulence regulon responsive to extracytoplasmic conditions. In conclusion, DsbA plays a crucial, multifaceted role in the pathogenesis of E. carotovora subsp. atroseptica.
Authors:
Sarah J Coulthurst; Kathryn S Lilley; Peter E Hedley; Hui Liu; Ian K Toth; George P C Salmond
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-18
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  283     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-25     Completed Date:  2008-10-03     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  23739-53     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom.
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE10647
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Catalysis
Disulfides / metabolism
Mutation
Pectobacterium carotovorum / enzymology*,  genetics
Plant Diseases / genetics,  microbiology
Protein Disulfide-Isomerases / genetics,  metabolism*
Proteome / genetics,  metabolism
Regulon / genetics
Solanum tuberosum / microbiology
Virulence Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Disulfides; 0/Proteome; 0/Virulence Factors; EC 5.3.4.1/Protein Disulfide-Isomerases
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