Document Detail


Drying rate and dehydrin synthesis associated with abscisic acid-induced dehydration tolerance in Spathoglottis plicata orchidaceae protocorms.
MedLine Citation:
PMID:  11847254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dehydration tolerance of in vitro orchid protocorms was investigated under controlled drying conditions and after abscisic acid (ABA) pretreatment. Protocorms were obtained by germinating seeds on Murashige and Skoog (MS) medium containing 10% (v/v) coconut water, 2% (w/v) sucrose and 0.8% (w/v) agar, and were dehydrated in relative humidities (RH) ranging from 7% to 93% at 25 degrees C. The critical water content of dehydration tolerance was determined, using the electrolyte leakage method. Drying rate affected the critical water content. Slow drying under high RH conditions achieved the greatest tolerance to dehydration. ABA pretreatment decreased the drying rate of protocorms, and increased dehydration tolerance. Improved tolerance to dehydration after ABA treatment was correlated with the effect of ABA on drying rate of protocorms. When critical water content of protocorms dried under different RH was plotted as a function of actual drying rate, no significant difference in tolerance to dehydration was observed between ABA-treated and control protocorms. ABA pretreatment and dehydration of orchid protocorms induced the synthesis of dehydrin, especially under the slow drying conditions. ABA pretreatment also promoted dry matter accumulation such as carbohydrates and soluble proteins and increased the concentration of K(+) and Na(+) ions in protocorms. The ABA-induced decrease in drying rate was correlated with lower osmotic potential, the enhanced maturity of protocorms and the accumulation of dehydrin in protocorms during pretreatment.
Authors:
Xing-Jun Wang; Chiang-Shiong Loh; Hock-Hin Yeoh; Wendell Q Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of experimental botany     Volume:  53     ISSN:  0022-0957     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-02-15     Completed Date:  2002-05-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  551-8     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / pharmacology*
Acclimatization / drug effects,  physiology
Blotting, Western
Calcium / analysis
Carotenoids / analysis
Chlorophyll / analysis
Heat-Shock Proteins / biosynthesis,  drug effects
Orchidaceae / drug effects,  growth & development,  metabolism*
Osmotic Pressure
Plant Growth Regulators / pharmacology*
Plant Proteins / biosynthesis*,  drug effects
Plant Structures / drug effects,  growth & development,  metabolism*
Potassium / analysis
Sodium / analysis
Water / metabolism*
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Plant Growth Regulators; 0/Plant Proteins; 134711-03-8/dehydrin proteins, plant; 1406-65-1/Chlorophyll; 21293-29-8/Abscisic Acid; 36-88-4/Carotenoids; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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