| Drug metabolite heterogeneity in cultured single cells profiled by pico-trapping direct mass spectrometry. | |
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MedLine Citation:
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PMID: 22583577 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aim: We investigated the heterogeneity of tafluprost metabolism in primary human hepatocytes at a single-cell level by live single-cell mass spectrometry (MS). Materials & methods: Picoliter volumes of cytoplasm were analyzed by nano-electrospray ionization MS in order to obtain single-cell metabolite profiles. The subcellular components of a single tafluprost-treated human hepatocyte were isolated and the single-cell metabolite profile was compared with those of traditional bulk hepatocyte analysis. Results: In the bulk hepatocyte analysis, liquid chromatography-MS showed the averaged metabolism of tafluprost to tafluprost acid (TA) and β-oxidized metabolites. However, live single-cell MS showed that tafluprost metabolism varied among individual cells. In addition, there was significant variation in the quantities of TA and a major metabolite, dinor-TA, among cells, whereas there was no significant variation in 7-ethoxycoumarin metabolism. Conclusion: Thus, live single-cell MS successfully detected the heterogeneity of drug metabolism in individual living hepatocytes. Original submitted 12 May 2011; Revised submitted 6 February 2012. |
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Authors:
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Yasufumi Fukano; Naohiro Tsuyama; Hajime Mizuno; Sachiko Date; Mikihisa Takano; Tsutomu Masujima |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-14 |
Journal Detail:
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Title: Nanomedicine (London, England) Volume: - ISSN: 1748-6963 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101278111 Medline TA: Nanomedicine (Lond) Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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