Document Detail


Drug induction of hepatic glutathione S-transferases in male and female rats*.
MedLine Citation:
PMID:  808217     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The induction of the glutathione S-transferases by phenobarbital and polycyclic hydrocarbons was studied in male and female rats. Administration of phenobarbital resulted in 60-80% increase in S-aryl and S-aralkyl enzyme specific activities, whereas the S-epoxide and S-alkyl activities were increased by 30-40%. In following the sequence of induction, the former two activities were noted to reach peak activities before an increase in the latter two activities was observed. Both 3-methylcholanthrene and 3,4-benzopyrene were shown toi nduce these four enzymic activities, although without the discrimination between pairs of activities noted with phenobarbital. No change in Km accompanied the increase in Vmax. after induction by drugs, and no change occurred in Ki for sulphobromophthalein inhibition. Significantly lower enzyme specific activities were found for three of the activities studied in female rats but no difference was observed in the S-alkyltransferase activity. However, the proportional increase in the enzymic activities in response to phenobarbital was the same in males and females. These studies demonstrate the drug induction of a group of cytosolic drug-metabolizing enzymes as well as the identification of sex differences in these activities.
Authors:
N Kaplowitz; J Kuhlekamp; G Clifton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  146     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1975 Feb 
Date Detail:
Created Date:  1975-11-07     Completed Date:  1975-11-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  351-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzopyrenes / pharmacology
Enzyme Induction
Female
Glutathione Transferase / metabolism
Haplorhini
Humans
Kinetics
Liver / drug effects,  enzymology*
Male
Methylcholanthrene / pharmacology
Phenobarbital / pharmacology*
Polycyclic Compounds / pharmacology*
Sex Factors
Transferases / metabolism*
Chemical
Reg. No./Substance:
0/Benzopyrenes; 0/Polycyclic Compounds; 50-06-6/Phenobarbital; 56-49-5/Methylcholanthrene; EC 2.-/Transferases; EC 2.5.1.18/Glutathione Transferase
Comments/Corrections

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