Document Detail


Drug-induced modulation of carcinoembryonic antigen (CEA) expression in neoplastic cells from a patient with rectal cancer.
MedLine Citation:
PMID:  11277324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treatment with 5-fluorouracil (5-FU) or recombinant interferon-gamma (IFN), alone or in combination, was found to increase carcinoembryonic antigen (CEA) expression in several carcinoma cell lines. In this study we examined the in vitro effect of these agents on CEA expression of tumor cells, obtained from a patient operated for rectal cancer. The results showed that exposure of cancer cells to 5-FU or to IFN resulted in increased CEA levels in terms of percentage of CEA-positive cells and mean fluorescence values, as indicated by FACS analysis. However, drug combination did not induce CEA expression higher than that provided by single agents alone. Treatment with 5-FU or with IFN produced a reduction of the total number of viable cells. Moreover, Western blot analysis revealed that exposure of cancer cells to each drug was followed by a substantial increase of the total cellular CEA content. On the contrary, 5-FU in combination with IFN did not increase the expression of the antigen more than that obtained by single agents. Noteworthy, exposure of CEA-negative cells from adjacent normal rectal tissue to both agents alone or in combination, did not result in CEA induction. In conclusion, the present results suggest new approaches aimed at (a) increasing the sensitivity of diagnostic procedures based on detection of CEA-positive tumor cells; (b) facilitating the recognition of CEA-positive cancer cells by immune responses induced by anti-CEA peptide vaccines.
Authors:
D Cappelletti; A Cardillo; E Bonanno; S P Prete; G Cucchiara; M Turriziani; J W Greiner; A Cottarelli; E Breda; A Aquino; E Bonmassar; L De Vecchis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of experimental & clinical cancer research : CR     Volume:  19     ISSN:  0392-9078     ISO Abbreviation:  J. Exp. Clin. Cancer Res.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-03-29     Completed Date:  2001-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8308647     Medline TA:  J Exp Clin Cancer Res     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  467-9     Citation Subset:  IM    
Affiliation:
Dept. of Neuroscience, University Tor Vergata, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Carcinoembryonic Antigen / blood,  metabolism*
Female
Flow Cytometry
Fluorouracil / pharmacology*
Humans
Interferon-gamma, Recombinant / pharmacology*
Rectal Neoplasms / metabolism*,  pathology,  surgery
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Carcinoembryonic Antigen; 0/Interferon-gamma, Recombinant; 51-21-8/Fluorouracil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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