| Drug cytotoxicity assay for African trypanosomes and Leishmania species. | |
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MedLine Citation:
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PMID: 7561203 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In this study, a spectrophotometric assay was developed and validated for measuring the cytotoxicity of test compounds against axenically cultured bloodstream-form Trypanosoma brucei (African trypanosomes) and promastigotes of Leishmania donovani. Enzymatic hydrolysis of p-nitrophenyl phosphate, monitored by a microtiter plate reader, is a reliable surrogate for parasite cell counts. The assay is simple, inexpensive, and highly reproducible. The coefficient of variation for EC50 values is < 10% for determinations obtained over several months. This method permits the rapid screening of candidates for much-needed new drugs against these parasites. |
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Authors:
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A L Bodley; M W McGarry; T A Shapiro |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 172 ISSN: 0022-1899 ISO Abbreviation: J. Infect. Dis. Publication Date: 1995 Oct |
Date Detail:
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Created Date: 1995-11-03 Completed Date: 1995-11-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1157-9 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2185, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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analysis Amphotericin B / pharmacology Animals Camptothecin / pharmacology Diminazene / analogs & derivatives, pharmacology Drug Evaluation, Preclinical / methods* Leishmania donovani / drug effects* Pentamidine / pharmacology Trypanocidal Agents / pharmacology* Trypanosoma brucei brucei / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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AI-28855/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Trypanocidal Agents; 100-33-4/Pentamidine; 1397-89-3/Amphotericin B; 536-71-0/Diminazene; 7689-03-4/Camptothecin; 908-54-3/diminazene aceturate; EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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