Document Detail


Drug cytotoxicity assay for African trypanosomes and Leishmania species.
MedLine Citation:
PMID:  7561203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In this study, a spectrophotometric assay was developed and validated for measuring the cytotoxicity of test compounds against axenically cultured bloodstream-form Trypanosoma brucei (African trypanosomes) and promastigotes of Leishmania donovani. Enzymatic hydrolysis of p-nitrophenyl phosphate, monitored by a microtiter plate reader, is a reliable surrogate for parasite cell counts. The assay is simple, inexpensive, and highly reproducible. The coefficient of variation for EC50 values is < 10% for determinations obtained over several months. This method permits the rapid screening of candidates for much-needed new drugs against these parasites.
Authors:
A L Bodley; M W McGarry; T A Shapiro
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  172     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-11-03     Completed Date:  1995-11-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1157-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2185, USA.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / analysis
Amphotericin B / pharmacology
Animals
Camptothecin / pharmacology
Diminazene / analogs & derivatives,  pharmacology
Drug Evaluation, Preclinical / methods*
Leishmania donovani / drug effects*
Pentamidine / pharmacology
Trypanocidal Agents / pharmacology*
Trypanosoma brucei brucei / drug effects*
Grant Support
ID/Acronym/Agency:
AI-28855/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Trypanocidal Agents; 100-33-4/Pentamidine; 1397-89-3/Amphotericin B; 536-71-0/Diminazene; 7689-03-4/Camptothecin; 908-54-3/diminazene aceturate; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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