Document Detail


Drug Delivery Characteristics of the Progenitor Bronchial Epithelial Cell Line VA10.
MedLine Citation:
PMID:  23135824     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE: To determine the integrity and permeability properties of the immortalized human VA10 bronchial epithelial cell line for its suitability as an in vitro drug permeation model. METHODS: Cells were grown under liquid-covered culture (LCC) or air-liquid interface (ALI) culture, characterized using electron microscopy and immunostaining. Integrity was measured using transepithelial electrical resistance (TER) and permeability of fluorescein sodium (Flu-Na). General permeability was established with dextrans and model drugs and P-glycoprotein (P-gp) function determined with bidirectional flux of rhodamine-123. RESULTS: ALI culture resulted in 2-3 cell layers with differentiation towards ciliated cells but LCC showed undifferentiated morphology. VA10 cells formed TJ, with higher TER in LCC than ALI (∼2500 vs. ∼1200 Ω*cm(2)) and Flu-Na permeability ∼1-2 × 10(-7) cm/s. ALI cultured cells expressed P-gp and distinguished between compounds depending on lipophilicity and size, consistent with previous data from Calu-3 and 16HBE14o-cell lines. CONCLUSIONS: ALI cultured cell layers capture the in vivo-like phenotype of bronchial epithelium and form functional cell barrier capable of discriminating between compounds depending on physiochemical properties. The VA10 cell line is an important alternative to previously published cell lines and a relevant model to study airway drug delivery in vitro.
Authors:
Berglind Eva Benediktsdóttir; Ari Jón Arason; Skarphédinn Halldórsson; Thórarinn Gudjónsson; Már Másson; Olafur Baldursson
Related Documents :
23514434 - Silver-based nanoparticles induce apoptosis in human colon cancer cells mediated throug...
23330784 - Gold nanoparticles cellular toxicity and recovery: adipose derived stromal cells.
23535374 - Oxidized silicon nanoparticles for radiosensitization of cancer and tissue cells.
24586454 - Monocyte chemotactic protein 1 (mcp-1) modulates pro-survival signaling to promote prog...
25450844 - The rate of hypo-osmotic challenge influences regulatory volume decrease (rvd) and mech...
10481084 - Anionic polymers of bacillus subtilis cell wall modulate the folding rate of secreted p...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Pharmaceutical research     Volume:  -     ISSN:  1573-904X     ISO Abbreviation:  Pharm. Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Faculty of Pharmaceutical Sciences, School of Health Sciences, University of Iceland, Hofsvallagata 53, 107, Reykjavik, Iceland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Extraction of PLGA-Microencapsulated Proteins Using a Two-Immiscible Liquid Phases System Containing...
Next Document:  The Impact of Glycosylation on the Pharmacokinetics of a TNFR2:Fc Fusion Protein Expressed in Glycoe...