Document Detail

Drosulfakinin activates CCKLR-17D1 and promotes larval locomotion and escape response in Drosophila.
MedLine Citation:
PMID:  22885328     Owner:  NLM     Status:  MEDLINE    
Neuropeptides are ubiquitous in both mammals and invertebrates and play essential roles in regulation and modulation of many developmental and physiological processes through activation of G-protein-coupled-receptors (GPCRs). However, the mechanisms by which many of the neuropeptides regulate specific neural function and behaviors remain undefined. Here we investigate the functions of Drosulfakinin (DSK), the Drosophila homolog of vertebrate neuropeptide cholecystokinin (CCK), which is the most abundant neuropeptide in the central nervous system. We provide biochemical evidence that sulfated DSK-1 and DSK-2 activate the CCKLR-17D1 receptor in a cell culture assay. We further examine the role of DSK and CCKLR-17D1 in the regulation of larval locomotion, both in a semi-intact larval preparation and in intact larvae under intense light exposure. Our results suggest that DSK/CCKLR-17D1 signaling promote larval body wall muscle contraction and is necessary for mediating locomotor behavior in stress-induced escape response.
Xu Chen; Jonathan Peterson; Ronald J Nachman; Barry Ganetzky
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-08-13
Journal Detail:
Title:  Fly     Volume:  6     ISSN:  1933-6942     ISO Abbreviation:  Fly (Austin)     Publication Date:    2012 Oct-Dec
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-06-21     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  101470897     Medline TA:  Fly (Austin)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  290-7     Citation Subset:  IM    
Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI, USA.
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MeSH Terms
Arrestins / analysis
Drosophila / genetics,  metabolism,  physiology*
Drosophila Proteins / metabolism*
Escape Reaction / physiology*
Green Fluorescent Proteins / analysis
Larva / physiology
Locomotion / physiology
Neuropeptides / genetics,  metabolism,  physiology*
Oligopeptides / genetics,  metabolism,  physiology*
Receptors, Cholecystokinin / metabolism*
Signal Transduction
Grant Support
Reg. No./Substance:
0/Arrestins; 0/CCKLR-17D1 protein, Drosophila; 0/Drosophila Proteins; 0/Ligands; 0/Neuropeptides; 0/Oligopeptides; 0/Receptors, Cholecystokinin; 0/beta-arrestin; 117457-90-6/drosulfakinin 1; 147336-22-9/Green Fluorescent Proteins

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