Document Detail


Drosophila stathmin: a microtubule-destabilizing factor involved in nervous system formation.
MedLine Citation:
PMID:  11854423     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stathmin is a ubiquitous regulatory phosphoprotein, the generic element of a family of neural phosphoproteins in vertebrates that possess the capacity to bind tubulin and interfere with microtubule dynamics. Although stathmin and the other proteins of the family have been associated with numerous cell regulations, their biological roles remain elusive, as in particular inactivation of the stathmin gene in the mouse resulted in no clear deleterious phenotype. We identified stathmin phosphoproteins in Drosophila, encoded by a unique gene sharing the intron/exon structure of the vertebrate stathmin and stathmin family genes. They interfere with microtubule assembly in vitro, and in vivo when expressed in HeLa cells. Drosophila stathmin expression is regulated during embryogenesis: it is high in the migrating germ cells and in the central and peripheral nervous systems, a pattern resembling that of mammalian stathmin. Furthermore, RNA interference inactivation of Drosophila stathmin expression resulted in germ cell migration arrest at stage 14. It also induced important anomalies in nervous system development, such as loss of commissures and longitudinal connectives in the ventral cord, or abnormal chordotonal neuron organization. In conclusion, a single Drosophila gene encodes phosphoproteins homologous to the entire vertebrate stathmin family. We demonstrate for the first time their direct involvement in major biological processes such as development of the reproductive and nervous systems.
Authors:
Sylvie Ozon; Antoine Guichet; Olivier Gavet; Siegfried Roth; André Sobel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biology of the cell     Volume:  13     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-20     Completed Date:  2002-06-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  698-710     Citation Subset:  IM    
Affiliation:
Institut National de la Santé et de la Recherche Médicale U440-Université Pierre et Marie Curie, Institut du Fer à Moulin, 75005 Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Drosophila / embryology,  physiology*
Exons / genetics
Hela Cells
Humans
Microtubule Proteins*
Microtubules / physiology*
Molecular Sequence Data
Nervous System / embryology*
Phosphoproteins / physiology*
Stathmin
Transfection
Chemical
Reg. No./Substance:
0/Microtubule Proteins; 0/Phosphoproteins; 0/STMN1 protein, human; 0/Stathmin
Comments/Corrections

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