| The Drosophila ortholog of the endolysosomal membrane protein, endolyn, regulates cell proliferation. | |
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MedLine Citation:
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PMID: 16924678 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endolyn (CD164) is a sialomucin that regulates the proliferation, adhesion, and migration of human haematopoietic stem and progenitor cells. This molecule is predominately localized in endocytotic compartments, where it may contribute to endolysosomal biogenesis and trafficking. In order to more closely define the function of endolyn from an evolutionary view-point, we first analyzed endolyn orthologs in species ranging from insects, fish, and birds to mammals. The predicted molecular structures of the endolyn orthologs from these species are well conserved, particularly with respect to significant O-linked glycosylation of the extracellular domain, and the high degree of amino acid similarities within their transmembrane and cytoplasmic domains, with the latter possessing the lysosomal target signal, YXXphi. Focusing on Drosophila, our studies showed that the subcellular distribution of endolyn in non-polarized Drosophila S2 cells resembles that of its human counterpart in hematopoietic cells, with its predominant localization being within intracellular vesicles, while a small fraction occurs on the cell surface. Both Y --> A and L --> A mutations in the YHTL motif perturbed the normal subcellular distribution of Drosophila endolyn. Interestingly, embryonic and early larval development was often arrested in endolyn-deficient Drosophila mutants. This may partly be due to the role of endolyn in regulating cell proliferation, since knock-down of endolyn expression in S2 cells resulted in up to 50% inhibition of cell growth, with a proportion of cells undergoing apoptosis. Taken together, these results demonstrate that endolyn is an evolutionarily conserved sialomucin fundamentally involved in cell proliferation in both the human and Drosophila melanogaster. |
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Authors:
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Guang-Qian Zhou; Youyi Zhang; David J P Ferguson; Sa Chen; Asa Rasmuson-Lestander; Frederick C Campbell; Suzanne M Watt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 99 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-02 Completed Date: 2007-05-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1380-96 Citation Subset: IM |
Copyright Information:
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2006 Wiley-Liss, Inc. |
Affiliation:
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Centre for Cancer Research and Cell Biology, Queen's University of Belfast, Belfast, UK. g.zhou@qub.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antigens, CD164 / genetics, metabolism* Cell Line Cell Proliferation* Computational Biology Drosophila Proteins / genetics, metabolism* Drosophila melanogaster / genetics, metabolism Humans Microscopy, Immunoelectron Molecular Sequence Data Phenotype RNA, Double-Stranded / genetics, metabolism Recombinant Fusion Proteins / genetics, metabolism Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD164; 0/Drosophila Proteins; 0/RNA, Double-Stranded; 0/Recombinant Fusion Proteins |
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