| Drosophila lysyl oxidases Dmloxl-1 and Dmloxl-2 are differentially expressed and the active DmLOXL-1 influences gene expression and development. | |
| | |
MedLine Citation:
|
PMID: 15811848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Mammalian lysyl oxidase (LOX) is essential for the catalysis of lysyl-derived cross-links in fibrillar collagens and elastin in the extracellular matrix and has also been implicated in cell motility, differentiation, and tumor cell invasion. The active LOX has been shown to translocate to the nuclei of smooth muscle cells and regulate chromatin structure and transcription. It is difficult to interpret the role of the LOX protein as it is co-expressed with other members of the LOX amine oxidase family in most mammalian cells. To investigate the function of the LOX proteins, we have characterized the Drosophila lysyl oxidases Dmloxl-1 and Dmloxl-2. We present the gene, domain structure, and expression pattern of Dmloxl-1 and Dmloxl-2 during development. In early development, only Dmloxl-1 was expressed, which allowed functional studies. We have expressed Dmloxl-1 in S2 cells and determined that it is a catalytically active enzyme, inhibited by beta-amino-proprionitrile (BAPN), a specific LOX inhibitor. We localized DmLOXL-1 in the nuclei in embryos and in adult salivary gland cells in the nuclei, cytoplasm, and cell surface, using immunostaining and a DmLOXL-1 antibody. To address the biological function of Dmloxl-1, we raised larvae under BAPN inhibitory conditions and over-expressed Dmloxl-1 in transgenic Drosophila. DmLOXL-1 inhibition resulted in developmental delay and a shift in sex ratio; over-expression in the w(m4) variegating strain increased drosopterin production, demonstrating euchromatinization. Our previous data on the transcriptional down-regulation of seven ribosomal genes and the glue gene under inhibitory conditions and the current results collectively support a nuclear role for Dmloxl-1 in euchromatinization and gene regulation. |
| | |
Authors:
|
Janos Molnar; Zsuzsanna Ujfaludi; Sheri F T Fong; John A Bollinger; Girma Waro; Ben Fogelgren; David M Dooley; Matyas Mink; Katalin Csiszar |
Related Documents
:
|
15946238 - Induction of connective tissue growth factor expression by sphingosylphosphorylcholine ... 15316508 - Leukotriene d4 stimulates collagen production from myofibroblasts transformed by tgf-beta. 17173248 - Glomerular expression of ctgf, tgf-beta 1 and type iv collagen in diabetic nephropathy. 12739618 - Differential expression of type xii collagen in developing chicken metatarsal tendons. 22050548 - Expression of the interleukin-10 signaling pathway genes in individuals with down syndr... 1706258 - The differential regulation by glucagon and growth hormone of insulin-like growth facto... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. Date: 2005-04-04 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 280 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2005 Jun |
Date Detail:
|
Created Date: 2005-06-13 Completed Date: 2005-07-19 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 22977-85 Citation Subset: IM |
Affiliation:
|
Cardiovascular Research Center, John A. Burns School of Medicine, University of Hawaii, Honolulu, 96822, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/AC007802; AC007803; AC008287; AC009349; AI260913; AJ295625; AJ295626; BT001394; M94054 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Active Transport, Cell Nucleus Amino Acid Sequence Aminopropionitrile / pharmacology Animals Animals, Genetically Modified Blotting, Northern Catalysis Cell Differentiation Cell Line Cell Membrane / metabolism Cell Movement Cell Nucleus / metabolism Chromatin / chemistry, metabolism Chromosome Mapping Collagen / chemistry Cytoplasm / metabolism DNA Primers / chemistry DNA, Complementary / metabolism Down-Regulation Drosophila melanogaster Elastin / chemistry Euchromatin / metabolism Extracellular Matrix / metabolism Gene Expression Regulation, Developmental* Gene Expression Regulation, Enzymologic* Genome Immunohistochemistry Microscopy, Confocal Models, Genetic Molecular Sequence Data Muscle, Smooth / cytology Polymerase Chain Reaction Protein Structure, Tertiary Protein-Lysine 6-Oxidase / biosynthesis*, chemistry* RNA, Messenger / metabolism Recombinant Proteins / chemistry Saliva / metabolism Salivary Glands / metabolism Sequence Homology, Amino Acid Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
|
AR47713/AR/NIAMS NIH HHS; GM27659/GM/NIGMS NIH HHS; RR03061/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Chromatin; 0/DNA Primers; 0/DNA, Complementary; 0/Euchromatin; 0/RNA, Messenger; 0/Recombinant Proteins; 151-18-8/Aminopropionitrile; 9007-34-5/Collagen; 9007-58-3/Elastin; EC 1.4.3.13/Protein-Lysine 6-Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Respiratory gated radiotherapy: the 4D radiotherapy
Next Document: An amino-terminal motif functions as a second nuclear export sequence in BRCA1.