Document Detail


Drosophila homologs of mammalian TNF/TNFR-related molecules regulate segregation of Miranda/Prospero in neuroblasts.
MedLine Citation:
PMID:  17139248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During neuroblast (NB) divisions, cell fate determinants Prospero (Pros) and Numb, together with their adaptor proteins Miranda (Mira) and Partner of Numb, localize to the basal cell cortex at metaphase and segregate exclusively to the future ganglion mother cells (GMCs) at telophase. In inscuteable mutant NBs, these basal proteins are mislocalized during metaphase. However, during anaphase/telophase, these mutant NBs can partially correct these earlier localization defects and redistribute cell fate determinants as crescents to the region where the future GMC "buds" off. This compensatory mechanism has been referred to as "telophase rescue". We demonstrate that the Drosophila homolog of the mammalian tumor-necrosis factor (TNF) receptor-associated factor (DTRAF1) and Eiger (Egr), the homolog of the mammalian TNF, are required for telophase rescue of Mira/Pros. DTRAF1 localizes as an apical crescent in metaphase NBs and this apical localization requires Bazooka (Baz) and Egr. The Mira/Pros telophase rescue seen in inscuteable mutant NBs requires DTRAF1. Our data suggest that DTRAF1 binds to Baz and acts downstream of Egr in the Mira/Pros telophase rescue pathway.
Authors:
Huashan Wang; Yu Cai; William Chia; Xiaohang Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-30
Journal Detail:
Title:  The EMBO journal     Volume:  25     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-13     Completed Date:  2007-02-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  5783-93     Citation Subset:  IM    
Affiliation:
Drosophila Neurobiology Lab, Institute of Molecular and Cell Biology, Proteos Building, 61 Biopolis Drive, Singapore 138673, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carrier Proteins
Cell Cycle Proteins / metabolism*
Cell Lineage
Cell Polarity
Chromosome Segregation*
Cytoskeletal Proteins / metabolism
Drosophila / cytology,  embryology,  metabolism*
Drosophila Proteins / metabolism*
Embryo, Nonmammalian / cytology,  embryology
Intracellular Signaling Peptides and Proteins / metabolism
Juvenile Hormones / metabolism
Mammals
Membrane Proteins / metabolism
Metaphase
Mutation / genetics
Nerve Tissue Proteins / metabolism*
Neurons / cytology*
Nuclear Proteins / metabolism*
Protein Transport
Receptors, Tumor Necrosis Factor / metabolism*
Sequence Homology
TNF Receptor-Associated Factor 1 / metabolism
Telophase
Transcription Factors / metabolism*
Tumor Necrosis Factors / metabolism*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cell Cycle Proteins; 0/Cytoskeletal Proteins; 0/Drosophila Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Juvenile Hormones; 0/Membrane Proteins; 0/Mira protein, Drosophila; 0/Nerve Tissue Proteins; 0/Nuclear Proteins; 0/Receptors, Tumor Necrosis Factor; 0/TNF Receptor-Associated Factor 1; 0/Transcription Factors; 0/Tumor Necrosis Factors; 0/bazooka protein, Drosophila; 0/eiger protein, Drosophila; 0/insc protein, Drosophila; 0/numb protein, Drosophila; 0/partner of Numb protein, Drosophila; 0/pros protein, Drosophila
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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