Document Detail


Drosophila genome-scale screen for PAN GU kinase substrates identifies Mat89Bb as a cell cycle regulator.
MedLine Citation:
PMID:  15737938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although traditional organism-based mutational analysis is powerful in identifying genes involved in specific biological processes, limitations include incomplete coverage and time required for gene identification. Biochemical screens using cell transfection or yeast two-hybrid methods are rapid, but they are limited by cDNA library quality. The recent establishment of "uni-gene sets" has made it feasible to biochemically screen an organism's entire genome. Radiolabeled protein pools prepared from the Drosophila Gene Collection were used in a Drosophila in vitro expression cloning ("DIVEC") screen for substrates of PAN GU kinase, which is crucial for S-M embryonic cell cycles. Ablation of one identified substrate, Mat89Bb, by RNAi produces a polyploid phenotype similar to that of pan gu mutants. Xenopus embryos injected with Mat89Bb morpholinos arrest with polyploid nuclei, and Mat89Bb RNAi in HeLa cells gives rise to multinucleated cells. Thus, Mat89Bb plays an evolutionarily conserved role as a crucial regulator of both cell cycle and development.
Authors:
Laura A Lee; Ethan Lee; Michael A Anderson; Leah Vardy; Emilios Tahinci; Siraj M Ali; Helena Kashevsky; Matt Benasutti; Marc W Kirschner; Terry L Orr-Weaver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental cell     Volume:  8     ISSN:  1534-5807     ISO Abbreviation:  Dev. Cell     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-01     Completed Date:  2005-05-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  435-42     Citation Subset:  IM    
Affiliation:
Whitehead Institute, Cambridge, Massachusetts 02142, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / physiology*
Cloning, Molecular
Drosophila / embryology,  enzymology
Drosophila Proteins / genetics,  metabolism*
Embryo, Nonmammalian / cytology,  enzymology*
Embryonic Development / physiology*
Genome*
Hela Cells
Humans
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Substrate Specificity / genetics
Xenopus / embryology,  metabolism
Grant Support
ID/Acronym/Agency:
GM39341/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; EC 2.7.1.-/Pan gu protein, Drosophila; EC 2.7.11.1/Protein-Serine-Threonine Kinases

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