Document Detail

Drosophila activating transcription factor-2 is involved in stress response via activation by p38, but not c-Jun NH(2)-terminal kinase.
MedLine Citation:
PMID:  15788564     Owner:  NLM     Status:  MEDLINE    
Activating transcription factor (ATF)-2 is a member of the ATF/cAMP response element-binding protein family of transcription factors, and its trans-activating capacity is enhanced by stress-activated protein kinases such as c-Jun NH(2)-terminal kinase (JNK) and p38. However, little is known about the in vivo roles played by ATF-2. Here, we identified the Drosophila homologue of ATF-2 (dATF-2) consisting of 381 amino acids. In response to UV irradiation and osmotic stress, Drosophila p38 (dp38), but not JNK, phosphorylates dATF-2 and enhances dATF-2-dependent transcription. Consistent with this, injection of dATF-2 double-stranded RNA (dsRNA) into embryos did not induce the dorsal closure defects that are commonly observed in the Drosophila JNK mutant. Furthermore, expression of the dominant-negative dp38 enhanced the aberrant wing phenotype caused by expression of a dominant-negative dATF-2. Similar genetic interactions between dATF-2 and the dMEKK1-dp38 signaling pathway also were observed in the osmotic stress-induced lethality of embryos. Loss of dATF-2 in Drosophila S2 cells by using dsRNA abrogated the induction of 40% of the osmotic stress-induced genes, including multiple immune response-related genes. This indicates that dATF-2 is a major transcriptional factor in stress-induced transcription. Thus, dATF-2 is critical for the p38-mediated stress response.
Yuji Sano; Hiroshi Akimaru; Tomoo Okamura; Tomoko Nagao; Masahiro Okada; Shunsuke Ishii
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Publication Detail:
Type:  Journal Article     Date:  2005-03-23
Journal Detail:
Title:  Molecular biology of the cell     Volume:  16     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-03     Completed Date:  2005-11-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2934-46     Citation Subset:  IM    
Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305-0074, Japan.
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MeSH Terms
Amino Acid Sequence
Blotting, Western
Cells, Cultured
Conserved Sequence
Drosophila / cytology,  embryology*,  genetics
Embryo, Nonmammalian
Enzyme Activation
Gene Expression Regulation, Developmental*
Genes, Insect
Genes, Reporter
Glutathione Transferase / metabolism
In Situ Hybridization
JNK Mitogen-Activated Protein Kinases*
Luciferases / metabolism
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis
Osmolar Concentration
Protein Structure, Tertiary
RNA, Messenger / metabolism
Recombinant Fusion Proteins / chemistry,  metabolism
Sequence Homology, Amino Acid
Transcriptional Activation
p38 Mitogen-Activated Protein Kinases / metabolism*
Reg. No./Substance:
0/RNA, Messenger; 0/Recombinant Fusion Proteins; EC 1.13.12.-/Luciferases; EC Transferase; EC Mitogen-Activated Protein Kinases; EC Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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