Document Detail


The Drosophila TRPL ion channel shares a Rab-dependent translocation pathway with rhodopsin.
MedLine Citation:
PMID:  21507505     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The Drosophila visual transduction cascade is embedded in the rhabdomeres of photoreceptor cells and culminates in the opening of the two ion channels, TRP and TRPL. TRPL translocates from the rhabdomeres to the cell body upon illumination and vice versa when flies are kept in the dark. Here, we studied the mechanisms underlying the light-dependent internalization of TRPL. Co-localization of TRPL and rhodopsin in endocytic particles revealed that TRPL is internalized by a vesicular transport pathway that is also utilized, at least partially, for rhodopsin endocytosis. TRPL internalization is attenuated under light conditions that result in a high rate of rhodopsin internalization and is highest in orange light that result in very little rhodopsin internalization. In line with a canonical vesicular transport pathway, we found that rab proteins, Rab5 and RabX4, are required for the internalization of TRPL into the cell body. Our results provide insight into stimulus-dependent internalization of a prominent member of the TRP superfamily.
Authors:
Claudia Oberegelsbacher; Carina Schneidler; Olaf Voolstra; Alexander Cerny; Armin Huber
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-18
Journal Detail:
Title:  European journal of cell biology     Volume:  -     ISSN:  1618-1298     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier GmbH. All rights reserved.
Affiliation:
Department of Biosensorics, Institute of Physiology, University of Hohenheim, 70599 Stuttgart, Germany.
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