Document Detail


Drosophila S2 cells secrete wingless on exosome-like vesicles but the wingless gradient forms independently of exosomes.
MedLine Citation:
PMID:  23035643     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wingless acts as a morphogen in Drosophila wing discs, where it specifies cell fates and controls growth several cell diameters away from its site of expression. Thus, despite being acylated and membrane associated, Wingless spreads in the extracellular space. Recent studies have focussed on identifying the route that Wingless follows in the secretory pathway and determining how it is packaged for release. We have found that, in medium conditioned by Wingless-expressing Drosophila S2 cells, Wingless is present on exosome-like vesicles and that this fraction activates signal transduction. Proteomic analysis shows that Wingless-containing exosome-like structures contain many Drosophila proteins that are homologous to mammalian exosome proteins. In addition, Evi, a multipass transmembrane protein, is also present on exosome-like vesicles. Using these exosome markers and a cell-based RNAi assay, we found that the small GTPase Rab11 contributes significantly to exosome production. This finding allows us to conclude from in vivo Rab11 knockdown experiments, that exosomes are unlikely to contribute to Wingless secretion and gradient formation in wing discs. Consistent with this conclusion, extracellularly tagged Evi expressed from a Bacterial Artificial Chromosome is not released from imaginal disc Wingless-expressing cells.
Authors:
Karen Beckett; Solange Monier; Lucy Palmer; Cyrille Alexandre; Hannah Green; Eric Bonneil; Graca Raposo; Pierre Thibault; Roland Le Borgne; Jean-Paul Vincent
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-31
Journal Detail:
Title:  Traffic (Copenhagen, Denmark)     Volume:  14     ISSN:  1600-0854     ISO Abbreviation:  Traffic     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-17     Completed Date:  2013-06-21     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  100939340     Medline TA:  Traffic     Country:  England    
Other Details:
Languages:  eng     Pagination:  82-96     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Chromosomes, Artificial, Bacterial
Drosophila / metabolism*
Drosophila Proteins / genetics,  metabolism*
Exosomes / metabolism*
Imaginal Discs / cytology
Intracellular Signaling Peptides and Proteins / metabolism*
RNA, Small Interfering
Secretory Vesicles / metabolism
Wnt1 Protein / metabolism*
rab GTP-Binding Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
MC_U117584268//Medical Research Council; U117584268//Medical Research Council; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/RNA, Small Interfering; 0/Wnt1 Protein; 0/Wntless protein, Drosophila; 0/wg protein, Drosophila; EC 3.6.1.-/Rab11 protein, Drosophila; EC 3.6.1.-/rab GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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