Document Detail

Drosophila PAT1 is required for Kinesin-1 to transport cargo and to maximize its motility.
MedLine Citation:
PMID:  20630947     Owner:  NLM     Status:  MEDLINE    
Kinesin heavy chain (KHC), the force-generating component of Kinesin-1, is required for the localization of oskar mRNA and the anchoring of the nucleus in the Drosophila oocyte. These events are crucial for the establishment of the anterior-posterior and dorsal-ventral axes. KHC is also essential for the localization of Dynein and for all ooplasmic flows. Interestingly, oocytes without Kinesin light chain show no major defects in these KHC-dependent processes, suggesting that KHC binds its cargoes and is activated by a novel mechanism. Here, we shed new light on the molecular mechanism of Kinesin function in the germline. Using a combination of genetic, biochemical and motor-tracking studies, we show that PAT1, an APP-binding protein, interacts with Kinesin-1, functions in the transport of oskar mRNA and Dynein and is required for the efficient motility of KHC along microtubules. This work suggests that the role of PAT1 in cargo transport in the cell is linked to PAT1 function as a positive regulator of Kinesin motility.
Philippe Loiseau; Tim Davies; Lucy S Williams; Masanori Mishima; Isabel M Palacios
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-14
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  137     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-28     Completed Date:  2010-08-20     Revised Date:  2011-07-25    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2763-72     Citation Subset:  IM    
The Zoology Department, University of Cambridge, Cambridge, UK.
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MeSH Terms
Biological Transport
Carrier Proteins / genetics,  metabolism*
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster / genetics,  metabolism*
Kinesin / genetics,  metabolism*
Protein Biosynthesis
RNA, Messenger / metabolism
Grant Support
//Biotechnology and Biological Sciences Research Council; //Wellcome Trust
Reg. No./Substance:
0/Carrier Proteins; 0/Drosophila Proteins; 0/PAT1 protein, Drosophila; 0/RNA, Messenger; 0/oskar protein, Drosophila; EC 3.6.1.-/Kinesin; EC 3.6.1.-/kinesin-1 protein, Drosophila

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