Document Detail


Drosophila lin-52 acts in opposition to repressive components of the Myb-MuvB/dREAM complex.
MedLine Citation:
PMID:  22688510     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Drosophila melanogaster Myb-MuvB/dREAM complex (MMB/dREAM) participates in both the activation and repression of developmentally regulated genes and origins of DNA replication. Mutants in MMB subunits exhibit diverse phenotypes, including lethality, eye defects, reduced fecundity, and sterility. Here, we used P-element excision to generate mutations in lin-52, which encodes the smallest subunit of the MMB/dREAM complex. lin-52 is required for viability, as null mutants die prior to pupariation. The generation of somatic and germ line mutant clones indicates that lin-52 is required for adult eye development and for early embryogenesis via maternal effects. Interestingly, the maternal-effect embryonic lethality, larval lethality, and adult eye defects could be suppressed by mutations in other subunits of the MMB/dREAM complex. These results suggest that a partial MMB/dREAM complex is responsible for the lethality and eye defects of lin-52 mutants. Furthermore, these findings support a model in which the Lin-52 and Myb proteins counteract the repressive activities of the other members of the MMB/dREAM complex at specific genomic loci in a developmentally controlled manner.
Authors:
Peter W Lewis; Debashis Sahoo; Cuiyun Geng; Maren Bell; Joseph S Lipsick; Michael R Botchan
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2012-06-11
Journal Detail:
Title:  Molecular and cellular biology     Volume:  32     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-30     Completed Date:  2012-10-16     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3218-27     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, Berkeley, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle Proteins / genetics,  metabolism*
Chromatography, Gel
Crosses, Genetic
DNA Replication
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster
E2F2 Transcription Factor / genetics,  metabolism*
Female
Gene Expression Regulation, Developmental*
Male
Microscopy, Fluorescence / methods
Models, Biological
Models, Genetic
Mutation
Photoreceptor Cells, Invertebrate / metabolism
Proto-Oncogene Proteins c-myb / genetics,  metabolism*
RNA Interference
Transcription Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 CA128836/CA/NCI NIH HHS; R01CA128836/CA/NCI NIH HHS; R37 CA030490/CA/NCI NIH HHS; R37CA030490/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Drosophila Proteins; 0/E2F2 Transcription Factor; 0/E2f2 protein, Drosophila; 0/Lin-52 protein, Drosophila; 0/Mip120 protein, Drosophila; 0/Myb protein, Drosophila; 0/Proto-Oncogene Proteins c-myb; 0/Transcription Factors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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