| Drosophila CAF-1 regulates HP1-mediated epigenetic silencing and pericentric heterochromatin stability. | |
| | |
MedLine Citation:
|
PMID: 20663913 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Chromatin assembly factor 1 (CAF-1) was initially characterized as a histone deliver in the process of DNA-replication-coupled chromatin assembly in eukaryotic cells. Here, we report that CAF-1 p180, the largest subunit of Drosophila CAF-1, participates in the process of heterochromatin formation and functions to maintain pericentric heterochromatin stability. We provide evidence that Drosophila CAF-1 p180 plays a role in both classes of position effect variegation (PEV) and in the expression of heterochromatic genes. A decrease in the expression of Drosophila CAF-1 p180 leads to a decrease in both H3K9 methylation at pericentric heterochromatin regions and the recruitment of heterochromatin protein 1 (HP1) to the chromocenter of the polytene chromosomes. The artificial targeting of HP1 to a euchromatin location leads to the enrichment of Drosophila CAF-1 p180 at this ectopic heterochromatin, suggesting the mutual recruitment of HP1 and CAF-1 p180. We also show that the spreading of heterochromatin is compromised in flies that have reduced CAF-1 p180. Furthermore, reduced CAF-1 p180 causes a defect in the dynamics of heterochromatic markers in early Drosophila embryos. Together, these findings suggest that Drosophila CAF-1 p180 is an essential factor in the epigenetic control of heterochromatin formation and/or maintenance. |
| | |
Authors:
|
Hai Huang; Zhongsheng Yu; Shuaiqi Zhang; Xuehong Liang; Jianming Chen; Changqing Li; Jun Ma; Renjie Jiao |
Related Documents
:
|
23159463 - Genetic sequence variants are associated with both severity of lower urinary tract symp... 22776853 - Cryptic del/dup aberration of 60.6 mb at 5q15-5q21.3 predicting adult-onset leucodystro... 16075203 - Mapping a resistance gene in wheat cultivar yangfu 9311 to yellow mosaic virus, using m... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-27 |
Journal Detail:
|
Title: Journal of cell science Volume: 123 ISSN: 1477-9137 ISO Abbreviation: J. Cell. Sci. Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-05 Completed Date: 2010-12-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
|
Languages: eng Pagination: 2853-61 Citation Subset: IM |
Affiliation:
|
State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Drosophila / genetics* Drosophila Proteins / genetics*, metabolism Epigenesis, Genetic Gene Expression Regulation Gene Silencing Heterochromatin / genetics*, metabolism Repressor Proteins / genetics, metabolism Retinoblastoma-Binding Protein 4 / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/CAF1 protein, Drosophila; 0/Drosophila Proteins; 0/Heterochromatin; 0/Repressor Proteins; 0/Retinoblastoma-Binding Protein 4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Occludin is required for cytokine-induced regulation of tight junction barriers.
Next Document: Human G3BP1 interacts with beta-F1-ATPase mRNA and inhibits its translation.