| Droplet microfluidics for amplification-free genetic detection of single cells. | |
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MedLine Citation:
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PMID: 22842841 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe-target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used to detect 16S rRNA present in pathogenic cells. Initially the sensitivity and quantification abilities of the platform are tested using a synthetic target mimicking the actual expression level of 16S rRNA in single cells. The capability of the device to perform "sample-to-answer" pathogen detection of single cells is demonstrated using E. coli as a model pathogen. |
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Authors:
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Tushar D Rane; Helena C Zec; Chris Puleo; Abraham P Lee; Tza-Huei Wang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-30 |
Journal Detail:
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Title: Lab on a chip Volume: - ISSN: 1473-0189 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biomedical Engineering, Johns Hopkins University, Baltimore, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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