Document Detail


Droplet microfluidics for amplification-free genetic detection of single cells.
MedLine Citation:
PMID:  22842841     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe-target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used to detect 16S rRNA present in pathogenic cells. Initially the sensitivity and quantification abilities of the platform are tested using a synthetic target mimicking the actual expression level of 16S rRNA in single cells. The capability of the device to perform "sample-to-answer" pathogen detection of single cells is demonstrated using E. coli as a model pathogen.
Authors:
Tushar D Rane; Helena C Zec; Chris Puleo; Abraham P Lee; Tza-Huei Wang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-30
Journal Detail:
Title:  Lab on a chip     Volume:  12     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-16     Completed Date:  2012-12-20     Revised Date:  2014-04-24    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  3341-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / isolation & purification*,  metabolism
Fluorescence Resonance Energy Transfer
Microfluidics / instrumentation,  methods*
Nucleic Acid Hybridization
Peptide Nucleic Acids / chemistry,  metabolism
RNA, Ribosomal, 16S / metabolism
Grant Support
ID/Acronym/Agency:
R01 CA155305/CA/NCI NIH HHS; R01CA155305/CA/NCI NIH HHS; U54 CA151838/CA/NCI NIH HHS; U54CA151838/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Peptide Nucleic Acids; 0/RNA, Ribosomal, 16S
Comments/Corrections

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