Document Detail


Droplet-based microsystem for multi-step bioreactions.
MedLine Citation:
PMID:  20204702     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A droplet-based microfluidic platform was used to perform on-chip droplet generation, merging and mixing for applications in multi-step reactions and assays. Submicroliter-sized droplets can be produced separately from three identical droplet-generation channels and merged together in a single chamber. Three different mixing strategies were used for mixing the merged droplet. For pure diffusion, the reagents were mixed in approximately 10 min. Using flow around the stationary droplet to induce circulatory flow within the droplet, the mixing time was decreased to approximately one minute. The shortest mixing time (10 s) was obtained with bidirectional droplet motion between the chamber and channel, and optimization could result in a total time of less than 1 s. We also tested this on-chip droplet generation and manipulation platform using a two-step thermal cycled bioreaction: nested TaqMan PCR. With the same concentration of template DNA, the two-step reaction in a well-mixed merged droplet shows a cycle threshold of approximately 6 cycles earlier than that in the diffusively mixed droplet, and approximately 40 cycles earlier than the droplet-based regular (single-step) TaqMan PCR.
Authors:
Fang Wang; Mark A Burns
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Biomedical microdevices     Volume:  12     ISSN:  1572-8781     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-04-26     Completed Date:  2010-07-27     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  533-41     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
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MeSH Terms
Descriptor/Qualifier:
Analytic Sample Preparation Methods / instrumentation*
DNA / chemistry*
Equipment Design
Equipment Failure Analysis
Flow Injection Analysis / instrumentation*
Microfluidic Analytical Techniques / instrumentation*
Polymerase Chain Reaction / instrumentation*
Grant Support
ID/Acronym/Agency:
1-R01-EB006789-01A2/EB/NIBIB NIH HHS; 5-R01-AI049541-06/AI/NIAID NIH HHS; R01 AI049541-06/AI/NIAID NIH HHS; R01 EB006789-01A2/EB/NIBIB NIH HHS; R01 EB006789-03/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA
Comments/Corrections

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