Document Detail


Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms.
MedLine Citation:
PMID:  18482695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-throughput, cell-based assays require small sample volumes to reduce assay costs and to allow for rapid sample manipulation. However, further miniaturization of conventional microtiter plate technology is problematic due to evaporation and capillary action. To overcome these limitations, we describe droplet-based microfluidic platforms in which cells are grown in aqueous microcompartments separated by an inert perfluorocarbon carrier oil. Synthesis of biocompatible surfactants and identification of gas-permeable storage systems allowed human cells, and even a multicellular organism (C. elegans), to survive and proliferate within the microcompartments for several days. Microcompartments containing single cells could be reinjected into a microfluidic device after incubation to measure expression of a reporter gene. This should open the way for high-throughput, cell-based screening that can use >1000-fold smaller assay volumes and has approximately 500x higher throughput than conventional microtiter plate assays.
Authors:
Jenifer Clausell-Tormos; Diana Lieber; Jean-Christophe Baret; Abdeslam El-Harrak; Oliver J Miller; Lucas Frenz; Joshua Blouwolff; Katherine J Humphry; Sarah Köster; Honey Duan; Christian Holtze; David A Weitz; Andrew D Griffiths; Christoph A Merten
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemistry & biology     Volume:  15     ISSN:  1074-5521     ISO Abbreviation:  Chem. Biol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-16     Completed Date:  2008-08-05     Revised Date:  2008-09-16    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  427-37     Citation Subset:  IM    
Affiliation:
Institut de Science et d'Ingénierie Supramoléculaires, Université Louis Pasteur, Strasbourg Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / cytology*
Emulsions
Humans
Microfluidics / instrumentation*
Miniaturization
Grant Support
ID/Acronym/Agency:
//Medical Research Council
Chemical
Reg. No./Substance:
0/Emulsions
Comments/Corrections
Erratum In:
Chem Biol. 2008 Aug 25;15(8):875

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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