Document Detail

Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms.
MedLine Citation:
PMID:  18482695     Owner:  NLM     Status:  MEDLINE    
High-throughput, cell-based assays require small sample volumes to reduce assay costs and to allow for rapid sample manipulation. However, further miniaturization of conventional microtiter plate technology is problematic due to evaporation and capillary action. To overcome these limitations, we describe droplet-based microfluidic platforms in which cells are grown in aqueous microcompartments separated by an inert perfluorocarbon carrier oil. Synthesis of biocompatible surfactants and identification of gas-permeable storage systems allowed human cells, and even a multicellular organism (C. elegans), to survive and proliferate within the microcompartments for several days. Microcompartments containing single cells could be reinjected into a microfluidic device after incubation to measure expression of a reporter gene. This should open the way for high-throughput, cell-based screening that can use >1000-fold smaller assay volumes and has approximately 500x higher throughput than conventional microtiter plate assays.
Jenifer Clausell-Tormos; Diana Lieber; Jean-Christophe Baret; Abdeslam El-Harrak; Oliver J Miller; Lucas Frenz; Joshua Blouwolff; Katherine J Humphry; Sarah Köster; Honey Duan; Christian Holtze; David A Weitz; Andrew D Griffiths; Christoph A Merten
Related Documents :
11084875 - Perfusion system for studying peptide secretion from endocrine cells with high time res...
9125155 - Hypotonicity and thrombin activate taurine efflux in bc3h1 and c2c12 myoblasts that is ...
16347945 - Regulation of bacterioplankton production and cell volume in a eutrophic estuary.
11125225 - Aldosterone and nuclear volume cycling.
25055205 - Rotenone isolated from pachyrhizus erosus displays cytotoxicity and genotoxicity in k56...
21919645 - Comparative antimutagenic and anticancer activity of three fractions of black tea polyp...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemistry & biology     Volume:  15     ISSN:  1074-5521     ISO Abbreviation:  Chem. Biol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-16     Completed Date:  2008-08-05     Revised Date:  2008-09-16    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  427-37     Citation Subset:  IM    
Institut de Science et d'Ingénierie Supramoléculaires, Université Louis Pasteur, Strasbourg Cedex, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Caenorhabditis elegans / cytology*
Microfluidics / instrumentation*
Grant Support
//Medical Research Council
Reg. No./Substance:
Erratum In:
Chem Biol. 2008 Aug 25;15(8):875

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Joining the army of proteasome inhibitors.
Next Document:  Peptidoglycan and muropeptides from pathogens Agrobacterium and Xanthomonas elicit plant innate immu...