Document Detail


Drinking a hot blood meal elicits a protective heat shock response in mosquitoes.
MedLine Citation:
PMID:  21518875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mosquito's body temperature increases dramatically when it takes a blood meal from a warm-blooded, vertebrate host. By using the yellow fever mosquito, Aedes aegypti, we demonstrate that this boost in temperature following a blood meal prompts the synthesis of heat shock protein 70 (Hsp70). This response, elicited by the temperature of the blood meal, is most robust in the mosquito's midgut. When RNA interference is used to suppress expression of hsp70, protein digestion of the blood meal is impaired, leading to production of fewer eggs. We propose that Hsp70 protects the mosquito midgut from the temperature stress incurred by drinking a hot blood meal. Similar increases in hsp70 were documented immediately after blood feeding in two other mosquitoes (Culex pipiens and Anopheles gambiae) and the bed bug, Cimex lectularius, suggesting that this is a common protective response in blood-feeding arthropods.
Authors:
Joshua B Benoit; Giancarlo Lopez-Martinez; Kevin R Patrick; Zachary P Phillips; Tyler B Krause; David L Denlinger
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-04-25
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-11     Completed Date:  2011-07-15     Revised Date:  2011-11-10    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8026-9     Citation Subset:  IM    
Affiliation:
Department of Entomology and Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210, USA. joshua.benoit@yale.edu
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MeSH Terms
Descriptor/Qualifier:
Aedes / genetics,  physiology
Animals
Anopheles gambiae / genetics,  physiology
Bedbugs / genetics,  physiology
Blood
Body Temperature / physiology
Culex / genetics,  physiology
Culicidae / genetics,  physiology*
Female
HSP70 Heat-Shock Proteins / antagonists & inhibitors,  biosynthesis,  genetics
Heat-Shock Response / genetics,  physiology*
Hot Temperature
Insect Proteins / antagonists & inhibitors,  biosynthesis,  genetics
RNA Interference
Grant Support
ID/Acronym/Agency:
R01AI058279/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/Insect Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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