| Drift-free atomic force microscopy measurements of cell height and mechanical properties. | |
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MedLine Citation:
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PMID: 17411236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Atomic force microscopy (AFM) is used to study the morphological and mechanical properties of living cells. However, experiments performed over minutes to hours are subject to significant instrumental drift. The main sources of drift are the cantilever's geometrical asymmetry and bimorphic construction. We developed a simple software Stick-and-Move (SaM) routine for AFM that eliminates drift by continuously referencing the sample position to the substrate while acquiring force-distance curves. Control experiments show no drift over 15 min at an acquisition rate of 0.1 Hz. As a proof of concept, we applied the SaM to study the response of rat astrocytes to osmotic stress, observing dimensional and constitutive changes during volume regulation. |
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Authors:
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Chiara Spagnoli; Arthur Beyder; Stephen R Besch; Frederick Sachs |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Review of scientific instruments Volume: 78 ISSN: 0034-6748 ISO Abbreviation: Rev Sci Instrum Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-04-06 Completed Date: 2007-06-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0405571 Medline TA: Rev Sci Instrum Country: United States |
Other Details:
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Languages: eng Pagination: 036111 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, University at Buffalo, State University of New York, 3435 Main Street, 301 Cary Hall, Buffalo, NY 14214, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytes / cytology Cell Size* Cells, Cultured Mechanics Microscopy, Atomic Force / methods* Rats Research Design Software* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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