Document Detail


Dried matrix on paper disks: the next generation DBS microsampling technique for managing the hematocrit effect in DBS analysis.
MedLine Citation:
PMID:  22946918     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background: The hematocrit effect is a hurdle for successful introduction of the dried blood spot (DBS) in a regulated environment. Recently, attempts were taken to overcome the hematocrit effect by whole-cut DBS analysis. This paper presents the next-generation whole-cut DBS; dried matrix on paper disks (DMPD). Results: DMPD eliminated the hematocrit effect and demonstrated better accuracy and precision than regular DBS with partial punching. Observed accuracy and precision were 6.0 and 2.3% for DMPD, respectively, and -10.4 and 17.1%, for DBS, respectively. Conclusion: The DMPD technique performed better than regular DBS by eliminating the hematocrit effect related blood volume bias. Although this effect was not observed with DMPD, a systematic error of 6.0% was detected and further technical development of DMPD could improve the performance.
Authors:
Roland Jw Meesters; Jamie Zhang; Nick A van Huizen; Gero P Hooff; Rob A Gruters; Theo M Luider
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioanalysis     Volume:  4     ISSN:  1757-6199     ISO Abbreviation:  Bioanalysis     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-09-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101512484     Medline TA:  Bioanalysis     Country:  England    
Other Details:
Languages:  eng     Pagination:  2027-35     Citation Subset:  IM    
Affiliation:
Universidad de los Andes, Faculty of Sciences, Department of Chemistry, Carrera 1 No. 18A-10, Bogota, Colombia. rj.meesters@uniandes.edu.co ; rjwmeesters@gmail.com.
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