Document Detail


Dpp signaling promotes the cuboidal-to-columnar shape transition of Drosophila wing disc epithelia by regulating Rho1.
MedLine Citation:
PMID:  19366729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Morphogenesis is largely driven by changes in the shape of individual cells. However, how cell shape is regulated in developing animals is not well understood. Here, we show that the onset of TGFbeta/Dpp signaling activity correlates with the transition from cuboidal to columnar cell shape in developing Drosophila melanogaster wing disc epithelia. Dpp signaling is necessary for maintaining this elongated columnar cell shape and overactivation of the Dpp signaling pathway results in precocious cell elongation. Moreover, we provide evidence that Dpp signaling controls the subcellular distribution of the activities of the small GTPase Rho1 and the regulatory light chain of non-muscle myosin II (MRLC). Alteration of Rho1 or MRLC activity has a profound effect on apical-basal cell length. Finally, we demonstrate that a decrease in Rho1 or MRLC activity rescues the shortening of cells with compromised Dpp signaling. Our results identify a cell-autonomous role for Dpp signaling in promoting and maintaining the elongated columnar shape of wing disc cells and suggest that Dpp signaling acts by regulating Rho1 and MRLC.
Authors:
Thomas J Widmann; Christian Dahmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-14
Journal Detail:
Title:  Journal of cell science     Volume:  122     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-23     Completed Date:  2009-09-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1362-73     Citation Subset:  IM    
Affiliation:
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning
Cell Shape*
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster* / anatomy & histology,  physiology
Embryo, Nonmammalian / cytology,  physiology
Epithelial Cells* / cytology,  physiology
Myosin Light Chains / genetics,  metabolism
Myosin Type II / genetics,  metabolism
RNA Interference
Signal Transduction / physiology*
Transforming Growth Factor beta / genetics,  metabolism
Wing / anatomy & histology,  embryology
rho GTP-Binding Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Myosin Light Chains; 0/Transforming Growth Factor beta; 0/dpp protein, Drosophila; EC 3.6.1.-/Myosin Type II; EC 3.6.5.2/Rho1 protein, Drosophila; EC 3.6.5.2/rho GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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