| Doxorubicin increases intracellular hydrogen peroxide in PC3 prostate cancer cells. | |
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MedLine Citation:
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PMID: 16054588 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the effect of doxorubicin on the production of hydrogen peroxide by PC3 human prostate cancer cells, using a sensitive assay based on aminotriazole-mediated inhibition of catalase. PC3 cells exposed to increasing concentrations of doxorubicin had an increase in intracellular hydrogen peroxide that was concentration-dependent up to 1 microM doxorubicin. The apparent hydrogen peroxide concentration in the PC3 cells was 13 +/- 4 pM under basal steady-state conditions and increased to 51 +/- 13 pM after exposure to 1 microM doxorubicin for 30 min. The level of hydrogen peroxide in the medium as measured by Amplex Red did not increase as a result of doxorubicin treatment. PC3 cells overexpressing catalase were no more resistant to doxorubicin cytotoxicity as compared to non-transduced wild-type cells; therefore, the exact role of hydrogen peroxide in anthracycline cytotoxicity remains unproven. This study demonstrates that a specific oxidative event associated with the exposure of PC3 human prostate cancer cells to anthracyclines results in an increase in intracellular hydrogen peroxide. |
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Authors:
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Brett A Wagner; Crystal B Evig; Krzysztof J Reszka; Garry R Buettner; C Patrick Burns |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 440 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-15 Completed Date: 2005-11-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
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Languages: eng Pagination: 181-90 Citation Subset: IM |
Affiliation:
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Department of Medicine, The University of Iowa Carver College of Medicine and Holden Comprehensive Cancer Center, Iowa City, IA 52242, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amitrole
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pharmacology Antibiotics, Antineoplastic / pharmacology* Catalase / metabolism Dose-Response Relationship, Drug Doxorubicin / pharmacology* Drug Resistance, Neoplasm Enzyme Inhibitors / pharmacology Fluorescent Dyes Humans Hydrogen Peroxide / metabolism* Intracellular Fluid / drug effects*, metabolism Male Oxidative Stress Prostatic Neoplasms / pathology Reactive Oxygen Species Transduction, Genetic Tumor Cells, Cultured / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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CA66081/CA/NCI NIH HHS; P01 CA66081/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Enzyme Inhibitors; 0/Fluorescent Dyes; 0/Reactive Oxygen Species; 23214-92-8/Doxorubicin; 61-82-5/Amitrole; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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