Document Detail


Doxorubicin-incorporated nanoparticles composed of poly(ethylene glycol)-grafted carboxymethyl chitosan and antitumor activity against glioma cells in vitro.
MedLine Citation:
PMID:  20427160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, methoxy poly(ethylene glycol)-grafted carboxymethyl chitosan (CMCPEG) was synthesized to make nanoparticles with doxorubicin (DOX) by ion complex formation. Since DOX has positive amine groups, it can interact with the carboxymethyl group of CMCPEG. The particle size of DOX-incorporated nanoparticles of CMCPEG was < 300 nm and nanoparticles had spherical shapes at morphological observation, indicating that DOX/CMCPEG mixtures can form spherical nanoparticles. In a drug release study, higher drug content induced an extended release of drug. Drug release was significantly changed by the release media pH. DOX release was faster at an acidic pH than a neutral or basic pH. The antitumor activity of DOX-incorporated nanoparticles in vitro was tested with DOX-resistant C6 glioma cells. Nanoparticles showed increased cytotoxicity compared to DOX alone. These results suggest that DOX was unable to penetrate into cells and did not effectively inhibit cell proliferation. In contrast, nanoparticles can penetrate into cells and effectively inhibit cell proliferation. Observation of cells under red fluorescence confirmed these results, i.e., nanoparticle-treated C6 cells, unlike DOX-treated cells, had strong red fluorescence. Since DOX has strong red fluorescence, DOX-incorporated nanoparticles entered into the tumor cells more than DOX alone. As a result, we suggest that DOX-incorporated nanoparticles of CMCPEG are superior candidates for antitumor drug delivery.
Authors:
Young-Il Jeong; Shu-Guang Jin; In-Young Kim; Jian Pei; Min Wen; Tae-Young Jung; Kyung-Sub Moon; Shin Jung
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-03
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  79     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  149-55     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Department of Neurosurgery, Chonnam National University, Hwasun Hospital & Medical School, Gwangju, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibiotics, Antineoplastic / chemistry,  pharmacokinetics,  toxicity
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Chitosan / analogs & derivatives*,  chemistry
Doxorubicin / chemistry*,  pharmacokinetics,  toxicity
Glioma / metabolism,  pathology
Kinetics
Nanoparticles / chemistry*,  toxicity
Nanotechnology / methods
Polyethylene Glycols / chemistry*
Technology, Pharmaceutical / methods
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Polyethylene Glycols; 0/carboxymethyl-chitosan; 23214-92-8/Doxorubicin; 9012-76-4/Chitosan

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