Document Detail


Doxorubicin exerts cytotoxic effects through cell cycle arrest and Fas-mediated cell death.
MedLine Citation:
PMID:  19829019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Doxorubicin (DOX) is involved in the induction of DNA damage, inhibition of cell proliferation, impairment of mitochondria, and cell death. To determine the biological effects of DOX in murine lymphocytes, we analyzed cell proliferation, cell cycle status, and apoptosis in Ba/F3 and EL4 lymphoid cells. DOX treatment resulted in significant cellular morphological alteration with increased intracellular granularity and cell size. DOX inhibited cell proliferation through cell cycle arrest at the G(2)/M phase as well as by cell death. In addition, DOX treatment dramatically upregulated Fas expression and enhanced caspase activation to promote intracellular apoptotic signaling for cell death. Treatment with an agonistic antibody stimulated Fas and accelerated the cell death effects. In conclusion, we demonstrate that DOX induces cell cycle arrest and apoptosis by increased Fas expression and ultimately results in enhanced cell death.
Authors:
Hye-Sun Kim; Yong-Soo Lee; Dong-Ku Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-14
Journal Detail:
Title:  Pharmacology     Volume:  84     ISSN:  1423-0313     ISO Abbreviation:  Pharmacology     Publication Date:  2009  
Date Detail:
Created Date:  2009-11-05     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0152016     Medline TA:  Pharmacology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  300-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Affiliation:
Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA Stem Cell Institute, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibiotics, Antineoplastic / pharmacology*
Antigens, CD95 / metabolism*
Apoptosis / drug effects*
Caspases / metabolism
Cell Cycle / drug effects*
Cell Enlargement / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Doxorubicin / pharmacology*
Fas Ligand Protein / metabolism*
Lymphocytes / cytology,  drug effects
Mice
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Antigens, CD95; 0/Fas Ligand Protein; 23214-92-8/Doxorubicin; EC 3.4.22.-/Caspases

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