| Doxazosin reduces cell proliferation and increases collagen fibers in rat prostatic lobes. | |
| | |
MedLine Citation:
|
PMID: 18188599 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We investigated the effects of doxazosin (Dox), an alpha-adrenoceptor antagonist used clinically for the treatment of benign prostatic hyperplasia (BPH), on the rat prostatic complex by assessing structural parameters, collagen fiber content, cell proliferation, and apoptosis. Adult Wistar rats were treated with Dox (25 mg/kg per day), and the ventral (VP), dorsolateral, and anterior prostate (AP) regions of the prostate complex were excised at 3, 7, and 30 days after treatment. At 24 h before being killed, the rats were injected once with 5-bromodeoxyuridine (BrdU; thymidine analog) to label mitotically active cells. The prostates were weighed and processed for histochemistry, morphometry-stereology, immunohistochemistry for BrdU, Western blotting for proliferating cell nuclear antigen (PCNA), and the TUNEL reaction for apoptosis. Dox-treated prostate lobes at day 3 presented increased weight, an enlarged ductal lumen, low cubical epithelial cells, reduced epithelial folds, and stretched smooth muscle cells. However, at day 30, the prostates exhibited a weight reduction of approximately 20% and an increased area of collagen and reticular fibers in the stromal space. Dox also reduced epithelial cell proliferation and increased apoptosis in the three prostatic lobes. Western blotting for PCNA confirmed the reduction of cell proliferation by Dox, with the AP and VP being more affected than the dorsal prostate. Thus, Dox treatment alters epithelial cell behavior and prostatic tissue mechanical demand, inducing tissue remodeling in which collagen fibers assume a major role. |
| | |
Authors:
|
Luis A Justulin; Flavia K Delella; Sérgio L Felisbino |
Related Documents
:
|
15982869 - Androgen receptor down regulation by small interference rna induces cell growth inhibit... 3664479 - Frequency and distribution of estrogen receptor-positive cells in normal, nonlactating ... 3029159 - Sex hormone-binding globulin secretion by human hepatocarcinoma cells is increased by b... 9017419 - A novel gene therapy strategy for elimination of prostate carcinoma cells from human bo... 10212269 - Fibroblast growth factor-10. a second candidate stromal to epithelial cell andromedin i... 20479999 - Potential effects of chrysin on mda-mb-231 cells. 20877389 - Cell death/proliferation and alterations in glial morphology contribute to changes in d... 9421419 - Developmental and hormonal regulation of keratinocyte growth factor expression and acti... 7850799 - Differential effects of n-(4-hydroxyphenyl)retinamide and retinoic acid on neuroblastom... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-10 |
Journal Detail:
|
Title: Cell and tissue research Volume: 332 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2008 Apr |
Date Detail:
|
Created Date: 2008-03-19 Completed Date: 2008-07-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
|
Languages: eng Pagination: 171-83 Citation Subset: IM |
Affiliation:
|
Department of Cell Biology, Institute of Biology, State University of Campinas, Campinas, SP, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / drug effects Body Weight / drug effects Cell Proliferation / drug effects* Collagen / metabolism* Doxazosin / pharmacology* Epithelial Cells / cytology, drug effects In Situ Nick-End Labeling Male Organ Size / drug effects Proliferating Cell Nuclear Antigen / metabolism Prostate / cytology, drug effects*, metabolism Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
|
0/Proliferating Cell Nuclear Antigen; 74191-85-8/Doxazosin; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of Bayofly on specimens of Culicoides species when incubated in hair taken from the feet of ...
Next Document: Presence and localization of aquaporin-6 in rat parotid acinar cells.